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3.5.4.5: cytidine deaminase

This is an abbreviated version!
For detailed information about cytidine deaminase, go to the full flat file.

Word Map on EC 3.5.4.5

Reaction

2'-deoxycytidine
+
H2O
=
2'-deoxyuridine
+
NH3

Synonyms

activation-induced cytidine deaminase, AICDA, canine hepatic cyd deaminase, CDA, CDABcald, CDABpsy, CDABsub, CDase, CDD, CR deaminase, cyd deaminase, Cytidine aminohydrolase, cytidine deaminase, cytidine/2'-deoxycytidine aminohydrolase, cytosine nucleoside deaminase, DCD, dCDA, deoxycytidine deaminase, EC 3.5.4.14, mammalian deminase, T-CDA, yeast cytidine deaminase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.5 cytidine deaminase

Cloned

Cloned on EC 3.5.4.5 - cytidine deaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cdd gene cloned and amplified with pTK148, cloning vector pBR322, enzyme overexpression in Escherichia coli C600
-
cdd genes cloned and characterized
cDNA coding for AT-CDA1 amplified, subcloned into pTrc99-A, induced and expressed and overproduced in Escherichia coli SO5201
-
cDNA, PCR, vector pTrc99A, cloned and expressed in Escherichia coli DH5alpha
-
enzyme expression in Escherichia coli strain BL21
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta(DE3)pLysS cells
expression in Escherichia coli
expression of wild-type and mutant enzymes in Escherichia coli, complementation of enzyme enzyme-deficient strain by the wild-type enzyme and mutants Y60G, R68Q, and R68G, not by F33 and F137A mutants
-
functional expression of the human enzyme in murine fibroblast cell lines 3T3 NIH and 3T3-CD3-V5 cells, and in the murine lymphocytic leukemia cell line L1210
-
gene At-cda1, located on chromosome 2 PCR amplified, cloned and sequenced, expressed in Escherichia coli BL21(D3)
-
overexpression of wild-type enzyme and mutant enzymes R56A, R56Q and C53H/R56Q in Escherichia coli
SO268 harboring plasmid pSO143 containing the B. subtilis cytidine deaminase gene
-
yielding plasmid pAA5970
-