3.5.4.3: guanine deaminase
This is an abbreviated version!
For detailed information about guanine deaminase, go to the full flat file.
Word Map on EC 3.5.4.3
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3.5.4.3
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xanthine
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purine
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deamination
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uric
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agdas
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hypoxanthine
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anogenital
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8-azaguanine
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anus
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penis
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scrotum
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medicine
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cephalad
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deaminases
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drug development
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analysis
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food industry
- 3.5.4.3
- xanthine
- purine
-
deamination
-
uric
-
agdas
- hypoxanthine
-
anogenital
- 8-azaguanine
-
anus
-
penis
-
scrotum
- medicine
-
cephalad
- deaminases
- drug development
- analysis
- food industry
Reaction
Synonyms
Agdap, cypin, cytosolic PSD-95 interactor, E. coli guanine deaminase, GAH, GDA, GDEase, GUA, guaD, guanase, guanine aminase, Guanine aminohydrolase, guanine deaminase, GUD1, human guanine deaminase, KLLAOD9492, More, NE0047, p51-nedasin, YgfP
ECTree
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Reaction
Reaction on EC 3.5.4.3 - guanine deaminase
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guanine + H2O = xanthine + NH3
catalytic mechanism, quantum mechanical calculations using multilayered ONIOM and molecular dynamics study, the active-site residues of the enzyme do not affect the tautomeric state of guanine, Glu55 and Asp114 play important roles in proton shuttling in the reaction, proton transfer from a Zn-bound water to protonate Asp114, which can then transfer its proton to the N3 of the bound guanine, facilitating the nucleophilic attack on C2 of the guanine by the Zn-bound hydroxide to form a tetrahedral intermediate. Glu55 then transfers a proton from the Zn-hydroxide to the amino group of the reaction intermediate, ammonia leaves the active site, and xanthine is freed by the cleavage of the Zn-O2 bond
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