3.5.4.27: methenyltetrahydromethanopterin cyclohydrolase
This is an abbreviated version!
For detailed information about methenyltetrahydromethanopterin cyclohydrolase, go to the full flat file.
Word Map on EC 3.5.4.27
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3.5.4.27
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methanobacterium
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thermoautotrophicum
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archaea
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methane
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formyltransferase
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methanogenic
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formylmethanofuran
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methanogenesis
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methylenetetrahydromethanopterin
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formylmethanofuran:tetrahydromethanopterin
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dioxide
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kandleri
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methanosarcina
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methanopyrus
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methanotrophic
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donnelly
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marburg
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wolfe
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lyotropic
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monoxide
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hyperthermophilic
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barkeri
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archaeoglobus
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consortia
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tetrahydrofolate
- 3.5.4.27
-
methanobacterium
- thermoautotrophicum
- archaea
- methane
-
formyltransferase
-
methanogenic
- formylmethanofuran
-
methanogenesis
- methylenetetrahydromethanopterin
-
formylmethanofuran:tetrahydromethanopterin
- dioxide
- kandleri
-
methanosarcina
-
methanopyrus
-
methanotrophic
-
donnelly
- marburg
-
wolfe
-
lyotropic
-
monoxide
-
hyperthermophilic
- barkeri
- archaeoglobus
-
consortia
- tetrahydrofolate
Reaction
Synonyms
5,10-methenyltetrahydromethanopterin cyclohydrolase, CBH37751, cyclohydrolase, hydrolase, methenyltetrahydromethanopterin cyclo-, Mch, methenyl-H4MPT cyclohydrolase, methenyl-H4MPT+ cyclohydrolase, methenyltetrahydromethanopterin cyclohydrolase, N5,N10-methenyltetrahydromethanopterin cyclohydrolase
ECTree
3.5.4.27 methenyltetrahydromethanopterin cyclohydrolaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.5.4.27 - methenyltetrahydromethanopterin cyclohydrolase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
5-formyl-5,6,7,8-tetrahydromethanopterin
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
5-formyl-5,6,7,8-tetrahydromethanopterin
the active site is primarily built up by the segment 93:95, Arg183 and Glu186 that either interact with the catalytic water attacking 5,10-methenyl-5,6,7,8-tetrahydromethanopterin or with the formyl oxygen of 5-formyl-5,6,7,8-tetrahydromethanopterin. The catalytic function of the strictly conserved Arg183 and Glu186 is substantiated by the low enzymatic activities of the E186A, E186D, E186N, E186Q, R183A, R183Q, R183E, R183K, and R183E-E186Q variants. Glu186 most likely acts as a general base. Arg183 decisively influences the pKa value of Glu186 and the proposed catalytic water mainly by its positive charge. In addition, Glu186 appears to be also responsible for product specificity by donating a proton to the directly neighbored N10 tertiary amine of H4MPT. Thus, N10 becomes a better leaving group than N5 which implies the generation of 5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
5-formyl-5,6,7,8-tetrahydromethanopterin
enzyme residue Lys77 binds to the phosphate moiety of substrate moiety H4MPT
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
5-formyl-5,6,7,8-tetrahydromethanopterin
enzyme residue Lys77 binds to the phosphate moiety of substrate moiety H4MPT
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
r
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
?
-
in acetate-grown cells the cyclohydrolase level is lowered 2 to 3fold, in cultures growing on acetate supplemented with methanol or H2CO cyclohydrolase activity level increases 3 to 4fold
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-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
?
Methanosarcina barkeri Fusaro / DSM 804
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in acetate-grown cells the cyclohydrolase level is lowered 2 to 3fold, in cultures growing on acetate supplemented with methanol or H2CO cyclohydrolase activity level increases 3 to 4fold
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-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
?
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enzyme is involved in methanogenesis from CO2 and H2, which represents the energy metabolism
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-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
?
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enzyme is involved in the formation of methane from CO2 in Methanobacterium thermoautotrophicum
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-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
?
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the enzyme might have a catabolic function in the C1-unit metabolism
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-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
?
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
the enzyme might have a catabolic function in the C1-unit metabolism
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
r
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
r
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
Methanosarcina barkeri Fusaro / DSM 804
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
N10-formyl-5,6,7,8-tetrahydromethanopterin is the nonenzymatic reaction product
r
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
the methenyl derivative is favored over 5-formyltetrahydromethanopterin
-
r
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
N5-formyl-5,6,7,8-tetrahydromethanopterin
-
-
-
?
formyl-tetrahydromethanopterin
methenyl-tetrahydromethanopterin + H2O
-
-
-
-
r
formyl-tetrahydromethanopterin
methenyl-tetrahydromethanopterin + H2O
-
-
-
-
r
formyl-tetrahydromethanopterin
methenyl-tetrahydromethanopterin + H2O
-
substrate isolated and purified from Methanobacterium thermoautotrophicum, overview
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-
r
formyl-tetrahydromethanopterin
methenyl-tetrahydromethanopterin + H2O
-
-
-
-
r
formyl-tetrahydromethanopterin
methenyl-tetrahydromethanopterin + H2O
-
-
-
-
r
formyl-tetrahydromethanopterin
methenyl-tetrahydromethanopterin + H2O
-
-
-
-
r
formyl-tetrahydromethanopterin
methenyl-tetrahydromethanopterin + H2O
-
-
-
-
r
