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3.5.4.27: methenyltetrahydromethanopterin cyclohydrolase

This is an abbreviated version!
For detailed information about methenyltetrahydromethanopterin cyclohydrolase, go to the full flat file.

Word Map on EC 3.5.4.27

Reaction

5,10-methenyl-5,6,7,8-tetrahydromethanopterin
+
H2O
=
5-formyl-5,6,7,8-tetrahydromethanopterin

Synonyms

5,10-methenyltetrahydromethanopterin cyclohydrolase, CBH37751, cyclohydrolase, hydrolase, methenyltetrahydromethanopterin cyclo-, Mch, methenyl-H4MPT cyclohydrolase, methenyl-H4MPT+ cyclohydrolase, methenyltetrahydromethanopterin cyclohydrolase, N5,N10-methenyltetrahydromethanopterin cyclohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.27 methenyltetrahydromethanopterin cyclohydrolase

Purification

Purification on EC 3.5.4.27 - methenyltetrahydromethanopterin cyclohydrolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and ion exchange chromatography, native enzyme over 600fold from cell supernatant by ammonium sulfate fractionation and two steps of ion exchange chromatography, purification requires anaerobic conditions, the enzyme is completely soluble at 80% saturated ammonium sulfate
-