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3.5.4.27: methenyltetrahydromethanopterin cyclohydrolase

This is an abbreviated version!
For detailed information about methenyltetrahydromethanopterin cyclohydrolase, go to the full flat file.

Word Map on EC 3.5.4.27

Reaction

5,10-methenyl-5,6,7,8-tetrahydromethanopterin
+
H2O
=
5-formyl-5,6,7,8-tetrahydromethanopterin

Synonyms

5,10-methenyltetrahydromethanopterin cyclohydrolase, CBH37751, cyclohydrolase, hydrolase, methenyltetrahydromethanopterin cyclo-, Mch, methenyl-H4MPT cyclohydrolase, methenyl-H4MPT+ cyclohydrolase, methenyltetrahydromethanopterin cyclohydrolase, N5,N10-methenyltetrahydromethanopterin cyclohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.27 methenyltetrahydromethanopterin cyclohydrolase

Engineering

Engineering on EC 3.5.4.27 - methenyltetrahydromethanopterin cyclohydrolase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E186A
Vmax is 0.035% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 2.1fold lower compared to wild-type value
E186D
Vmax is 0.56% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.1fold higher compared to wild-type value
E186N
Vmax is 0.1% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.1fold lower compared to wild-type value
E186Q
nearly inactive mutant enhzyme
K94E
Vmax is 86% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.4fold lower compared to wild-type value
K94V
Vmax is 41% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.2fold lower compared to wild-type value
R183a
Vmax is 0.07% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.3fold lower compared to wild-type value
R183E
Vmax is 0.01% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 2.2fold lower compared to wild-type value
R183E/E186Q
no activity
R183K
Vmax is 15% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.3fold lower compared to wild-type value
R183Q
Vmax is 0.05% of wild-type activity, Km for 5,10-methenyl-5,6,7,8-tetrahydromethanopterin is 1.3fold lower compared to wild-type value