3.5.4.25: GTP cyclohydrolase II

This is an abbreviated version!
For detailed information about GTP cyclohydrolase II, go to the full flat file.

Reaction

Synonyms

3,4-dihydroxy-2-butanone 4-phosphate synthase, GCH II, GCH II/III, GCH-II, gch2, GCHII, GTP 7,8-8,9-dihydrolase (diphosphate-forming), GTP cyclohydrolase 2, GTP cyclohydrolase II, GTP cyclohydrolase-II, GTP-8-formylhydrolase, GTPCH II, GTPCH-II, guanosine triphosphate cyclohydrolase II, guanosine triphosphate cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase, guanosine-5'-triphosphate cyclohydrolase-II, More, NbRibA, ribA, ribA2, RIBIV, ToxB

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.4 In cyclic amidines

                3.5.4.25 GTP cyclohydrolase II

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Results	in table
31774	AA Sequence
2	Application
1	CAS Registry Number
8	Cloned(Commentary)
3	Crystallization (Commentary)
15	General Information
12	Inhibitors
2	kcat/KM [mM/s]
1	Ki Value [mM]
39	KM Value [mM]
13	Metals/Ions
10	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
30	Organism
12	Pathway
7	PDB ID
8	pH Optimum
2	pH Range
1	pI Value
32	Protein Variants
8	Purification (Commentary)
5	Reaction
4	Reaction Type
30	Reference
3	Source Tissue
14	Specific Activity [micromol/min/mg]
2	Storage Stability
39	Substrates and Products (Substrate)
3	Subunits
39	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Stability [°C]
35	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 3.5.4.25 - GTP cyclohydrolase II

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate + H2O

?

2 entries

2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate + 2 H2O

formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate

2 entries

2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate + H2O

2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate

Escherichia coli

-

formamide-type intermediate analogue

655443

-

-

?

8-oxo-dGTP + H2O

?

Escherichia coli

-

-

246734

-

-

?

dGTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phospho-deoxyribosylamino)pyrimidine + diphosphate

Escherichia coli

-

-

655443

-

-

?

GTP + 3 H2O

formate + 2,5-diamino-6-beta-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate

2 entries

GTP + 3 H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate

2 entries

GTP + H2O

2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?

Streptomyces coelicolor

O88011, Q9EWJ8, Q9KYI5

enzyme catalyzes the first step in the biosynthesis of riboflavin

696250

-

-

?

GTP + H2O

2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?

2 entries

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate

3 entries

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate

4 entries

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate

5 entries

GTP + H2O

formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate

3 entries

additional information

?

-

11 entries

2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate + H2O

?

Bacillus subtilis

-

-

711974

-

-

?

2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate + H2O

?

Escherichia coli

P0A7I7

-

734013

-

-

?

2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate + 2 H2O

formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate

Escherichia coli

-

compound does serve as a substrate but not as kinetically competent intermediate

656029

-

-

?

2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate + 2 H2O

formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate

Escherichia coli

-

formamide-type intermediate analogue

655443

-

-

?

GTP + 3 H2O

formate + 2,5-diamino-6-beta-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate

Escherichia coli

-

-

656006

spontaneous isomerization into the alpha-isomer, also the product can easily spontaneously decompose under formation of 2,4,5-triamino-4-(3H)-pyrimidone

-

?

GTP + 3 H2O

formate + 2,5-diamino-6-beta-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate

Escherichia coli

-

first committed step in the biosynthesis of riboflavin, overview

656006

-

-

?

GTP + 3 H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate

Helicobacter pylori

O08315

-

757510

-

-

?

GTP + 3 H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate

Helicobacter pylori ATCC 700392

O08315

-

757510

-

-

?

GTP + H2O

2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?

Streptomyces coelicolor

O88011, Q9EWJ8, Q9KYI5

-

696250

-

-

?

GTP + H2O

2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?

Streptomyces coelicolor

-

-

685065

the reaction product is exclusively formed by enzyme variant SCO 6655

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate

Escherichia coli

P0A7I7

-

734013

GMP is a second reaction product of GTP cyclohydrolase II. The ratio of the main product, 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine and GMP is produced at an approximate ratio of 10:1

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate

Mycobacterium tuberculosis

P9WHF1

-

726585

-

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate

Mycobacterium tuberculosis H37Rv

P9WHF1

-

726585

-

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate

Arabidopsis thaliana

-

-

246731

-

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate

Escherichia coli

-

-

209545, 246729, 246730, 246732, 246734

-

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate

Helicobacter pylori

-

-

246733

-

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate

Meyerozyma guilliermondii

-

-

209545

-

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate

Bacillus subtilis

-

-

675226, 711974

-

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate

Eremothecium ashbyi

A6MYZ0

-

712085

-

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate

Eremothecium ashbyi NRRL Y-1363

A6MYZ0

-

712085

-

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate

Escherichia coli

-

-

655443

-

-

?

GTP + H2O

formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate

Escherichia coli

-

first committed step in the biosynthesis of riboflavin, overview

655443

-

-

?

GTP + H2O

formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate

Escherichia coli

-

-

656029

GMP is also formed as a minor product

-

?

GTP + H2O

formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate

Escherichia coli

-

first committed step in the biosynthesis of riboflavin, overview

656029

-

-

?

GTP + H2O

formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate

Streptomyces coelicolor

-

-

685065

-

-

?

additional information

?

-

Bacillus subtilis

-

RibA is the rate limiting enzyme in an industrial riboflavin producing strain

675226

-

-

?

additional information

?

-

Bacillus subtilis

-

RibA is a bifunctional enzyme possessing 3,4-dihydroxy-2-butanone 4-phosphate synthase activity, EC 4.1.99.12, located in the N-terminal half of the protein and GTP cyclohydrolase II activity of the C-terminal domain, overview

675226

-

-

?

additional information

?

-

Escherichia coli

P0A7I7

GTP cyclohydrolase II is a slow GTPase

734013

-

-

?

additional information

?

-

Streptomyces coelicolor

O88011

enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II

696250

-

-

?

additional information

?

-

Streptomyces coelicolor

Q9EWJ8

enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II

696250

-

-

?

additional information

?

-

Streptomyces coelicolor

Q9KYI5

enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II

696250

-

-

?

additional information

?

-

Streptomyces coelicolor

-

enzyme may be involved in the biosynthesis of toxoflavin, which is produced from GTP in a series of steps that are likely initiated by GCH II

696250

-

-

?

additional information

?

-

Streptomyces coelicolor

O88011

substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency

696250

-

-

?

additional information

?

-

Streptomyces coelicolor

Q9EWJ8

substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency

696250

-

-

?

additional information

?

-

Streptomyces coelicolor

Q9KYI5

substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency

696250

-

-

?

additional information

?

-

Streptomyces coelicolor

-

substitution of Met with His leads to a protein that catalyzes the production of both 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate and 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate with equal efficiency

696250

-

-

?