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3.5.4.2: adenine deaminase

This is an abbreviated version!
For detailed information about adenine deaminase, go to the full flat file.

Word Map on EC 3.5.4.2

Reaction

adenine
+
H2O
=
hypoxanthine
+
NH3

Synonyms

aadA, AAH, Aah1p, AAur1117, ADA, ADase, ADE, adenase, adenine aminase, adenine deaminase, KLLAOE2317, MJ1459, NadA, Pa0148, Sgx9403e, Sgx9403g

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.2 adenine deaminase

Storage Stability

Storage Stability on EC 3.5.4.2 - adenine deaminase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, ammonium sulfate, for at least 2 months
-
4°C, 10 mM phoshate buffer, pH7.5, 20 mM 2-mercaptoethanol, 1-2 weeks, minimal losses
4°C, 25 mM Tris, 100 mM NaCl, 15% glycerol and pH 6.0, stable for a month
-
activity of purified protein is stable for 1-2 weeks when stored at 4°C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20°C with 50% (v/v) glycerol and at -80°C with 10-20% (v/v) glycerol or as freeze-dried powder
at 4°C in potassium buffer above a concentration of 10 mg/ml and in presence of 20% glycerol stable for 1-2 weeks, prolonged storage in solution at -20°C in presence of 50% glycerol and at -80°C in presence of 10-20% glycerol, or as freeze-dried powder