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3.5.4.16: GTP cyclohydrolase I

This is an abbreviated version!
For detailed information about GTP cyclohydrolase I, go to the full flat file.

Word Map on EC 3.5.4.16

Reaction

GTP
+
H2O
=
formate
 +
7,8-dihydroneopterin 3'-triphosphate

Synonyms

dihydroneopterin triphosphate synthase, FelE, folE, GCH, GCH-1, GCH1, GCHI, GCYH-IA, GCYH-IB, GTP 8-formylhydrolase, GTP CHase I, GTP cyclohydrolase, GTP cyclohydrolase 1, GTP cyclohydrolase I, GTP cyclohydrolase IA, GTP cyclohydrolase IB, GTP cyclohydrolase-1, GTP-CH, GTP-CH-I, GTP-CH1, GTP-cyclohydrolase 1, GTP-cyclohydrolase I, GTPCH, GTPCH I, GTPCH-1, GTPCH1, GTPCHI, guanosine 5'-triphosphate-cyclohydrolase I, guanosine triphosphate 8-deformylase, guanosine triphosphate cyclohydrolase, guanosine triphosphate cyclohydrolase 1, guanosine triphosphate cyclohydrolase I, guanosine-5'-triphosphate cyclohydrolase 1, hydrolase, guanosine triphosphate cyclo-, MptA, Punch, Punch protein

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.16 GTP cyclohydrolase I

Cloned

Cloned on EC 3.5.4.16 - GTP cyclohydrolase I

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into the yeast expression vector pVT103-U and introduced into yeast strain 971/6a
enzyme expression in Escherichia coli strain BL21(DE3)
expressed in a recombinant Escherichia coli strain
expressed in Escherichia coli B834(DE3) cells
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli BL21(DE3)RIL cells
expressed in Escherichia coli strain BL21-Codon Plus (DE3)-PIL
-
expressed in Escherichia coli, baby hamster kidney cells, and NIH-293 cells
-
expressed in HEK-293 cells
-
expressed in Mus musculus
-
expressed in Mus musculus microvascular endothelial cells
-
expressed in PC-12 cells
-
expression in Escherichia coli
expression of DELTA42-hGTP-CH-I in Escherichia coli M15
expression of GTPCH under the control of a 935-bp fragment of the mouse myosin heavy chain gene promoter
-
expression of His-tagged wild-type GTPCH as maltose-binding protein fusion protein in Escherichia coli, expression of a His-tagged GTPCH truncation mutant, devoid of 45 N-terminal amino acids, DELTA45-GTPCH, in HEK-293 cells
-
expression of the enzyme in Escherichia coli strain DH5alpha as maltose binding protein fusion protein
-
expression of the enzyme in transgenic Lycopersicon esculentum plants using the Agrobacterium tumefaciens transfection system
gene gch and adjacent regions, DNA and amino acid sequence determination and analysis, uncommon start codon TTG, ribosomal binding site, overexpression in Escherichia coli strains JM109 and BL21(DE3) as HAT-tagged enzyme, and expression in Mycobacterium smegmatis from plasmid
overexpressed as an N-terminally His6 tagged protein in Bl21(DE3) cells
overexpression of wild-type and mutant enzymes from plasmids in strain DH5alpha
-
transient co-expression of the enzyme with estrogen receptors in PC12 cells
-