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3.5.4.13: dCTP deaminase

This is an abbreviated version!
For detailed information about dCTP deaminase, go to the full flat file.

Word Map on EC 3.5.4.13

Reaction

dCTP
+
H2O
=
dUTP
 +
NH3

Synonyms

5-methyl-dCTP deaminase, DCD:DUT, dcdB, dCMP-dCTP deaminase, dCTP deaminase, dCTP deaminase:deoxyuridine triphosphatase, dCTP deaminase:dUTPase, deoxycytidine triphosphate deaminase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.13 dCTP deaminase

Engineering

Engineering on EC 3.5.4.13 - dCTP deaminase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E138A
E138D
site-directed mutagenesis, the mutant enzyme shows a 140fold reduction in kcat and altered dTTP inhibition compared to the wild-type enzyme
E138Q
no enzymic activity, no change in overall structure compared to wild-type
H121A
site-directed mutagenesis, inactive mutant, dTTP is bound to the active site of E138A, the region between Val120 and His125 is formed in a new conformation, the C-terminal fold is disordered
R115A
no enzymic activity, no change in overall structure compared to wild-type
R115Q
site-directed mutagenesis, inactive mutant
S111C
site-directed mutagenesis, inactive mutant
S111T
site-directed mutagenesis, the mutant enzyme shows a 30fold reduction in kcat and altered dTTP inhibition compared to the wild-type enzyme, modeling of the active site of the S111T enzyme, overview
V122G
site-directed mutagenesis, inactive mutant