3.5.4.13: dCTP deaminase

This is an abbreviated version!
For detailed information about dCTP deaminase, go to the full flat file.

Word Map on EC 3.5.4.13

Reaction

dCTP
+
H2O
=
dUTP
+
NH3

Synonyms

deoxycytidine triphosphate deaminase, 5-methyl-dCTP deaminase, dCTP deaminase, dCMP-dCTP deaminase, dCTP deaminase:dUTPase, dCTP deaminase:deoxyuridine triphosphatase, DCD:DUT, dcdB

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.13 dCTP deaminase

Crystallization

Crystallization on EC 3.5.4.13 - dCTP deaminase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with dTTP, hanging drop vapor diffusion method, using 200 mM lithium citrate and 20% (w/v) polyethylene glycol 4000
wild-type and mutant E138A, in complex with Mg2+ and dUTP, and dCTP
mutant E138A bound to dTTP and mutant H121A bound to dCTP, hanging drop vapour diffusion method, 3.7 mg/ml E138A or 5.1 mg/ml H121A in 20 mM magnesium chloride, 50 mM HEPES, pH 6.8, and 5 mM nucleotide, mixing of 0.002 ml of protein solution with an equal volume of reservoir solution containing 34% PEG 400, 0.2 mM MgCl2, and 0.1 m HEPES, pH 7.5, equilibration against 1 ml of mother liquor, room temperature, 1 week, X-ray diffraction structure determination and analysis at 2.6-2.7 A resolution
recombinant mutant E138D in complex with dUTP, hanging drop vapour diffusion method, 0.002 ml of 2 mg/ml protein in solution with 5 mM dUTP and 20 mM MgSO4 is mixed with 0.002 ml reservoir solution containing 27.5% PEG 400, 50 mM MgSO4 and 0.1 M HEPES, pH 7.5, equilibration against 0.5 ml of reservoir solution, room temeprature, crystal X-ray diffraction structure determination and analysis at 2.1 A resolution
apoenzyme in complex with dCTP and with dUTP