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3.5.3.9: allantoate deiminase

This is an abbreviated version!
For detailed information about allantoate deiminase, go to the full flat file.

Word Map on EC 3.5.3.9

Reaction

Allantoate
+
H2O
=
(S)-ureidoglycine
 +
NH3
 +
CO2

Synonyms

AAH, ADE2, ALAH, Allantoate amidinohydrolase, allantoate amidohydrolase, allantoate-degrading enzyme, ALLC, AtAAH

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.9 allantoate deiminase

Inhibitors

Inhibitors on EC 3.5.3.9 - allantoate deiminase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
21% inhibition at 1 mM
Acetohydroxamate
-
-
Allantoate
Borate
Cd2+
Streptococcus allantoicus
-
at pH 6.5
diethyldicarbonate
-
-
Fe2+
-
0.15 mM completely inhibits incubation mixture with Mn2+
fluoride
glycolate
-
50% activity loss with 0.06 mM glycolate for 10 min at 30°C; strong inhibitor, 50% inhibition at 0.06 mM
glyoxylate
-
50% activity loss with 0.02 mM glycolate for 10 min at 30°C; strong inhibitor, 50% inhibition at 0.02 mM
guanidine
-
10% and 80% activity loss with 2 M and 5 M L-guanidine pre-treatment for 10 min at 30°C
hydroxylamine
-
67% inhibition at 1 mM
iminourea
-
pre-treatment with 2mM or 5mM results in a loss of acitivity of 10% or 80% of activity
L-Asn
L-Asp
N-Acetylimidazole
-
5.6% activity loss with 10 mM n-acetylimidazole 10 min at 30°C
N-bromosuccinimide
-
100% activity loss with 10 mM n-bromosuccinimide for 10 min at 30°C; pre-treatment completely inhibits activity
NEM
-
15% inhibition at 5 mM
Ni2+
Streptococcus allantoicus
-
at pH 6.5
nitrate
-
inhibits the enzyme, overview
pyridoxal 5'-phosphate
-
pre-treatment with 10 mM completely inhibits activity
pyridoxal-5-phosphate
-
100% activity loss with 10 mM pyridoxal-5-phosphate for 10 min at 30°C
SDS
-
50% activity loss with 50 mM SDS pre-treatment for 10 min at 30°C; pre-treatment with 50 mM results in a loss of acitivity of 50% of activity
Urea
-
5% and 20% activity loss with 2 M and 5 M urea pre-treatment for 10 min at 30°C
additional information
-