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3.5.3.15: protein-arginine deiminase

This is an abbreviated version!
For detailed information about protein-arginine deiminase, go to the full flat file.

Word Map on EC 3.5.3.15

Reaction

protein L-arginine
+
H2O
=
protein L-citrulline
 +
NH3

Synonyms

deiminase, protein (arginine), HL-60 PAD, hPADI2, hPADI4, hPADVI, PAD, PAD 1, PAD 2, PAD 3, PAD 4, PAD II, PAD IV, PAD type 2, PAD-4, PAD-H19, PAD-R11, PAD-R4, PAD1, PAD2, PAD3, PAD4, PAD6, PADI1, Padi2, PADI3, PADI4, peptidyl arginine deiminas 4, peptidyl arginine deiminase, peptidyl arginine deiminase 2, peptidyl-arginine deiminase, peptidylarginine deiminase, peptidylarginine deiminase 1, peptidylarginine deiminase 2, peptidylarginine deiminase 3, peptidylarginine deiminase 4, peptidylarginine deiminase 6, peptidylarginine deiminase isoform VI, peptidylarginine deiminase IV, peptidylarginine deiminase type 4, Peptidylarginine deiminase type alpha, peptidylarginine deiminase type I, peptidylarginine deiminase type II, peptidylarginine deiminase type III, peptidylarginine deiminase type VI, peptidylarginine deiminase-4, PPAD, protein arginine deiminase, protein arginine deiminase 2, protein arginine deiminase 3, protein arginine deiminase 4

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.15 protein-arginine deiminase

Cloned

Cloned on EC 3.5.3.15 - protein-arginine deiminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3'-untranslated nucleotide sequence
-
expressed in Escherichia coli BL21 cells as a fusion protein with glutathione S-transferase
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli BL21-pLysS cells
-
expressed in Escherichia coli strain BL21(DE3)pLysS, COS-1 and HEK-293 cells
expressed in Escherichia coli strain BL21, HEK-293T, and MCF-7 cell lines
expressed in Mus musculus
expressed in Sf21 insect cells
expression analysis
expression in Escherichia coli
expression of PAD4
gene padi4, DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes
-
hPADI2 is expressed in Escherichia coli
-
hPADI4 is expressed in Escherichia coli
-
overexpression in Escherichia coli
overexpression of GST-tagged PAD4 mutant C645A
PAD2 and PAD4 from spleen, expression of N-terminally His-tagged enzymes in Escherichia coli strain BL21(DE3)
promoter sequence determination and anaylsis 195 base pairs upstream from the transcription initiation site, primer extension method and determination of minimal promoter sequence, the promoter activity depends on MFZ1 and Sp1/Sp3 binding to their specific sites