diverse multidrug resistant extended-spectrum beta-lactamase-producing Escherichia coli strains among uropathogens of pediatrics in north of Iran are isolated and tested by PCR for the presence or absence of CTX, TEM, SHV, GES, and VEB beta-lactamase genes, genotyping
DNA and amino acid sequence determination and analysis, PCR screening for MBL variants in cystic fibrosis patient isolates of Pseudomonas aeruginosa in Portugal, overview
gene blaC, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene blaCMY-2, DNA and amino acid sequence determination and analysis, genotyping of the bla gene in 14 pAmpC-producing Escherichia coli strains, 11 sequence types are found, genomic relationships analysis
gene blaIMP-1, quantitative expression analysis in 124 different strains, among metallo-beta-lactamase-producing Gram-negative rods in Japan, 84.45% are blaIMP-1 or blaIMP-2
gene blaIMP-10, DNA and amino acid sequence determination and analysis, promoter analysis, overexpression of metallo-beta-lactamase IMP-10 by the strong promoter of gene blaIMP-10 in Pseudomonas aeruginosa strain KG2505, overview. Genetic structure of the class 1 integron carrying the blaIMP-10 cassette, overview
gene blaIMP-15, the blaIMP-15 gene cassette is inserted in a plasmid-borne integron with a unique array of gene cassettes and named In95, genotyping for beta-lactamase genes in human isolates from a hospital in Mexico, overview. Genetic structure in strain 4677, overview, transformations of plasmid preparations of strains 4677 and 4663 by electroporation into Escherichia coli strain DH10B and Pseudomonas aeruginosa strain PU21
gene blaNDM-7, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strains BL21(DE3) and CS109, subcloning in Escherichia coli strain XL-1 Blue. Host Escherichia coli strain CS109 harbouring cloned blaNDM-7 displays a resistant status with over 60fold increased resistance compared to control strain CS109 for all penicillins, cephalosporin and carbapenems tested
gene blaSHV-14, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strains BL21(DE3) and CS109. On expression, the clone of wild-type SHV-14 aggravates the resistance of host by 60-500 folds against penicillin and cephalosporin groups of antibiotics, while the expression of mutated enzymes (especially E164A) substantially reduces the resistance level as compared to the wild-type, and the results are in synchrony with the estimated enzymatic efficiencies of wild-type and mutated proteins
gene blaVIM, genotyping for beta-lactamase genes in human isolates from a hospital in Mexico, overview. Genetic structure in strain 4677, overview, transformations of plasmid preparations of strains 4677 and 4663 by electroporation into Escherichia coli strain DH10B and Pseudomonas aeruginosa strain PU21
gene macQ, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain Origami 2(DE3). Recombinant plasmid (pMQ28) confers 2 to 16fold higher MIC values toward all six beta-lactam antibiotics in Escherichia coli Origami 2(DE3) than the strain with a control vector. This indicates that MacQ confers multiple beta-lactam antibiotic resistance on a host strain by hydrolyzing beta-lactam antibiotics
gene MSMEG_4455, DNA and amino acid sequence determination and analysis, recombinant ectopic expression of wild-type and mutant enzymes in beta-lactamase-deficient Escherichia coli mutant strain AM1OC-1, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
genes blaOXA-10, blaVIM-2, blaPSE-I, blaOXA-4, blaOXA-17, blaOXA-30, DNA and amino acid sequence determination and analysis, genotyping in human isolates and correlation of amikain resistance, overview
genes blaVIM1 and blaVIM-13, genetic structure and genotyping, VIM-13 is harbored in a class 1 integron, along with an A108T variant of the aminoglycoside-modifying enzyme encoding gene aacA4, which confers resistance to gentamicin and tobramycin
genotyping and screening of clinical isolates from human patients, sequencing of gene bla-VIM in MBl variant VIM-2, random amplified polymorphic DNA, RAPD, types, overview
identification of SHV-type extended spectrum beta-lactamase genes in Pseudomonas aeruginosa by PCR-restriction fragment length polymorphism and insertion site restriction-PCR, genotyping, overview
macrorestriction profiling of chromosomal DNA from 30 isolates and identification of two different clonal lineages, named cluster A, serotype O11, and cluster B, serotype O12, that produce VIM-1 and VIM-2 MBLs, respectively. The VIM-2-producing isolates from Italy carried identical, or very similar, allelic forms of the oprD gene, harbour a common class 1 integron, belong to the same multilocus sequence type, and show macrorestriction profiles that are related to those of the MBL-negative French strains, overview
screening for genes blaIMP-1, blaVIM and blaVIM-2 in clinical human isolates from a university hospital in Sichuan, China, genotyping and antimicrobial susceptibility, overview
3.5.2.6 beta-lactamase
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