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3.5.2.12: 6-aminohexanoate-cyclic-dimer hydrolase

This is an abbreviated version!
For detailed information about 6-aminohexanoate-cyclic-dimer hydrolase, go to the full flat file.

Word Map on EC 3.5.2.12

Reaction

1,8-Diazacyclotetradecane-2,9-dione
+
H2O
=
N-(6-aminohexanoyl)-6-aminohexanoate

Synonyms

6-aminohexanoate-cyclic- dimer hydrolase (Caulobacter crescentus gene CC1323), 6-aminohexanoate-cyclic- dimer hydrolase (Deinococcus radiodurans strain R1 gene DR0235), 6-aminohexanoate-cyclic-dimer hydrolase, Acd hydrolase, hydrolase, aminocaproate cyclic dimer, Nylon oligomers degrading enzyme EI, omega-laurolactam hydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.12 6-aminohexanoate-cyclic-dimer hydrolase

Engineering

Engineering on EC 3.5.2.12 - 6-aminohexanoate-cyclic-dimer hydrolase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C316A
-
18% of wild-type hydrolytic activity, decrease in the Km to approximately one-quarter the level of wild-type NylA, CD spectra similar to wild-type
C316D
-
1.5% of wild-type activity
C316E
-
1%% of wild-type activity
C316G
-
185% of wild-type activity
C316K
-
3.6% of wild-type activity
C316N
-
4.3% of wild-type activity
C316S
-
107% of wild-type activity
K72A
-
decreases the hydrolytic and esterolytic activities to undetectable levels
N125A
-
14% of wild-type hydrolytic activity, CD spectra similar to wild-type
S150A
-
decreases the hydrolytic and esterolytic activities to undetectable levels
S174A
-
decreases the hydrolytic and esterolytic activities to undetectable levels