3.5.2.12: 6-aminohexanoate-cyclic-dimer hydrolase
This is an abbreviated version!
For detailed information about 6-aminohexanoate-cyclic-dimer hydrolase, go to the full flat file.
Word Map on EC 3.5.2.12
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3.5.2.12
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6-aminohexanoate-dimer
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arthrobacter
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flavobacterium
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acidovorax
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compendium
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amides
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hydrolases
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hydrolysing
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sphingomonas
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man-made
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cupriavidus
- 3.5.2.12
-
6-aminohexanoate-dimer
- arthrobacter
- flavobacterium
- acidovorax
-
compendium
- amides
- hydrolases
-
hydrolysing
- sphingomonas
-
man-made
- cupriavidus
Reaction
Synonyms
6-aminohexanoate-cyclic- dimer hydrolase (Caulobacter crescentus gene CC1323), 6-aminohexanoate-cyclic- dimer hydrolase (Deinococcus radiodurans strain R1 gene DR0235), 6-aminohexanoate-cyclic-dimer hydrolase, Acd hydrolase, hydrolase, aminocaproate cyclic dimer, Nylon oligomers degrading enzyme EI, omega-laurolactam hydrolase
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Engineering
Engineering on EC 3.5.2.12 - 6-aminohexanoate-cyclic-dimer hydrolase
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C316A
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18% of wild-type hydrolytic activity, decrease in the Km to approximately one-quarter the level of wild-type NylA, CD spectra similar to wild-type
K72A
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decreases the hydrolytic and esterolytic activities to undetectable levels
N125A
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14% of wild-type hydrolytic activity, CD spectra similar to wild-type
S150A
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decreases the hydrolytic and esterolytic activities to undetectable levels
S174A
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decreases the hydrolytic and esterolytic activities to undetectable levels