3.5.1.9: arylformamidase

This is an abbreviated version!
For detailed information about arylformamidase, go to the full flat file.

Word Map on EC 3.5.1.9

3.5.1.9

organophosphorus

l-tryptophan

formylation

teratogen

n-formyl-l-kynurenine

teratogenesis

methylcarbamate

l-kynurenine

diazinon

parvulus

pyrimidinyls

kynurenic

xanthurenic

triesters

degradation

formyltransferase

crotonamide

Reaction

Synonyms

AFMID, Bna7p, FKF, formamidase I, formamidase II, formyl kynurenine formamidase, formylase, formylkynureninase, formylkynurenine formamidase, KFA, KFase, KynB, kynurenine formamidase, kynurine formamidase

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.1 In linear amides

3.5.1.9 arylformamidase

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Results	in table
15	Activating Compound
5226	AA Sequence
4	Application
1	CAS Registry Number
10	Cloned(Commentary)
1	Cofactor
4	Crystallization (Commentary)
3	Expression
23	General Information
4	General Stability
166	Inhibitors
4	kcat/KM [mM/s]
11	Ki Value [mM]
38	KM Value [mM]
7	Localization
11	Metals/Ions
53	Molecular Weight [Da]
73	Natural Substrates/ Products (Substrates)
71	Organism
12	Pathway
18	PDB ID
23	pH Optimum
5	pH Range
2	pH Stability
1	pI Value
4	Protein Variants
25	Purification (Commentary)
6	Reaction
1	Reaction Type
67	Reference
55	Source Tissue
27	Specific Activity [micromol/min/mg]
4	Storage Stability
105	Substrates and Products (Substrate)
7	Subunits
40	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]
1	Temperature Range [°C]
7	Temperature Stability [°C]
5	Turnover Number [1/s]

Purification

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Purification on EC 3.5.1.9 - arylformamidase

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4 entries

native enzyme from liver cytosol, recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by anion exchange chromatography, gel filtration, and nickel affinity chromatography, to high purity, recombinant wild-type enzyme 90fold

Mus musculus

Q8K4H1

670776

near homogeneity, chromatography techniques

Gallus gallus

209247

near homogeneity, chromatography techniques, isoelectric focusing

Rattus sp.

209250

partial

3 entries

recombinant enzyme from Escherichia coli by affinity chromatography, ion exchange chromatography, and gel filtration

Drosophila melanogaster

Q9VMC9

733287

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration

Streptomyces coelicolor

Q9X8R1

733183

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, cleavage of the tag by tobacco etch virus, followed by another step of nickel affinity chromatography, gel filtration of the eluate

3 entries

to homogeneity, chromatography steps

Rattus sp.

209258

to homogeneity, chromatography steps, isoelectric focusing

Rattus sp.

209257

to homogeneity, chromatography techniques

3 entries

to homogeneity, chromatography techniques, 2 forms

2 entries

to homogeneity, chromatography techniques, preparative disc gel electrophoresis

Gallus gallus

209256

to homogeneity: formamidase I, partial: formamidase II, chromatography techniques

Drosophila melanogaster

209251