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3.5.1.9: arylformamidase

This is an abbreviated version!
For detailed information about arylformamidase, go to the full flat file.

Word Map on EC 3.5.1.9

Reaction

N-formyl-L-kynurenine
+
H2O
=
formate
+
L-kynurenine

Synonyms

AFMID, Bna7p, FKF, formamidase I, formamidase II, formyl kynurenine formamidase, formylase, formylkynureninase, formylkynurenine formamidase, KFA, KFase, KynB, kynurenine formamidase, kynurine formamidase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.9 arylformamidase

Cloned

Cloned on EC 3.5.1.9 - arylformamidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and anlysis, expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain DH10B
expressed in Escherichia coli
expression of putative kynBAU in Escherichia coli
-
gene CG9542, cloning from larvae, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli
gene kynB or SCO3644, gene expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
gene kynB, recombinant expression of the His6-tagged enzyme in Escherichia coli K12 strain BL21(DE3)pLysS, codon optimization
gene kynB, sequence comparison, recombinant expression of the His6-tagged enzyme in Escherichia coli K12 strain BL21(DE3)pLysS, codon optimization
kynB gene, expression in Escherichia coli as N-terminal His6 tag fusions