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3.5.1.79: Phthalyl amidase

This is an abbreviated version!
For detailed information about Phthalyl amidase, go to the full flat file.

Reaction

A phthalylamide
+
H2O
=
Phthalic acid
+
a substituted amine

Synonyms

Amidase, phthalyl, o-Phthalyl amidase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.79 Phthalyl amidase

General Stability

General Stability on EC 3.5.1.79 - Phthalyl amidase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
pH-stability and temperature-stability is improved significantly by increasing ionic strength of the buffer
-
stability is dependent on the high ionic strength of the buffer
-
the enzyme stability is optimal at salt concentrations of 0.2 M and above
-
the substrate phthalimido carbacephem stablilizes the enzyme
-