3.5.1.78: Glutathionylspermidine amidase

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For detailed information about Glutathionylspermidine amidase, go to the full flat file.

Reaction

Synonyms

Amidase, glutathionylspermidine, Glutathionylspermidine amidohydrolase (spermidine-forming), Glutathionylspermidine amidohydrolase [spermidine-forming], Glutathionylspermidine synthetase/amidase, GSP amidase, Gsp synthetase/amidase, GspA, GspSA, GSS, trypanothione synthetase-amidase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.78 Glutathionylspermidine amidase

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Results	in table
348	AA Sequence
1	CAS Registry Number
6	Cloned(Commentary)
1	Cofactor
2	Crystallization (Commentary)
4	General Information
1	General Stability
3	IC50 Value
8	Inhibitors
7	KM Value [mM]
1	Metals/Ions
4	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
11	Organism
2	Pathway
15	PDB ID
1	pH Optimum
6	Protein Variants
4	Purification (Commentary)
3	Reaction
1	Reaction Type
11	Reference
1	Renatured (Commentary)
1	Specific Activity [micromol/min/mg]
17	Substrates and Products (Substrate)
3	Subunits
22	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Stability [°C]
1	Turnover Number [1/s]

Purification

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Purification on EC 3.5.1.78 - Glutathionylspermidine amidase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Escherichia coli

-

968

nickel affinity column chromatography

Escherichia coli

P0AES0

716863

recombinant enzyme

Crithidia fasciculata

-

649733

recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange chromatography and gel filtration

Escherichia coli

P0AES0

727627