Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.5.1.78: Glutathionylspermidine amidase

This is an abbreviated version!
For detailed information about Glutathionylspermidine amidase, go to the full flat file.

Word Map on EC 3.5.1.78

Reaction

glutathionylspermidine
+
H2O
=
glutathione
+
spermidine

Synonyms

Amidase, glutathionylspermidine, Glutathionylspermidine amidohydrolase (spermidine-forming), Glutathionylspermidine amidohydrolase [spermidine-forming], Glutathionylspermidine synthetase/amidase, GSP amidase, Gsp synthetase/amidase, GspA, GspSA, GSS, trypanothione synthetase-amidase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.78 Glutathionylspermidine amidase

Cloned

Cloned on EC 3.5.1.78 - Glutathionylspermidine amidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli, His6-tag
-
gene gsp, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene gss, phylogenetic and global transcriptome analyses, microarray studies comparing gss+ and DELTAgss strains of Escherichia coli show that a large number of genes are either upregulated (76 genes more than 3fold) or downregulated (35 genes more than 3fold) by the loss of the gss gene. Most significant categories of up-regulated genes include sulfur utilization, glutamine and succinate metabolism, polyamine and arginine metabolism, and purine and pyrimidine metabolism
mutant enzyme C59A is expressed in Escherichia coli BL21(DE3) cells