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3.5.1.70: aculeacin-A deacylase

This is an abbreviated version!
For detailed information about aculeacin-A deacylase, go to the full flat file.

Word Map on EC 3.5.1.70

Reaction

aculeacin A
+
H2O
=
palmitic acid
 +
deacyl-aculeacin A

Synonyms

AAC, aac1, aculeacin A acylase, AuAAC, cyclic lipopeptide acylase, deacylase, aculeacin A

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.70 aculeacin-A deacylase

Temperature Stability

Temperature Stability on EC 3.5.1.70 - aculeacin-A deacylase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
4 h, inactivation at pH 2, stable at pH 4-8
45
-
both soluble and immobilized enzyme apparently suffers no loss of enzymatic activity when stored at 45°C for at least 550 min
50
-
20 min, stable up to
60
-
24 h, 0.1 M phosphate buffer, pH 7, stable at
65
-
the recombinant immobilized enzyme is highly thermostable since it suffers no loss of activity at 65°C and pH 8.0 during 3 h
70
-
above, 0.1 M phosphate buffer, pH 7, rapid loss of activity
75
-
the recombinant immobilized enzyme is completely deactivated after its incubation during two hours at temperatures above 75°C
82
-
aculeacin A acylase is highly thermostable, with a midpoint transition temperature of 81.5°C
additional information
-
half-life of the soluble enzyme at 65°C is estimate, t1/2: 477 min