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3.5.1.65: theanine hydrolase

This is an abbreviated version!
For detailed information about theanine hydrolase, go to the full flat file.

Reaction

N5-Ethyl-L-glutamine
+
H2O
=
L-glutamate
 +
ethylamine

Synonyms

hydrolase, theanine, L-theanine hydrolase, L-theanine hydrolyzing enzyme, theanine ethylamidohydrolase, theanine hydrolytic enzyme, theanine hydrolyzing enzyme

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.65 theanine hydrolase

Inhibitors

Inhibitors on EC 3.5.1.65 - theanine hydrolase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetic acid
-
8 mM: 88% of activity compared to H2O
acidic amino acid
-
-
-
beta-Alanine
-
partial inhibition, 8 mM: 89% of activity compared to H2O
CuSO4
-
10 mM: strong inhibition
EDTA
-
10 mM: 30% inhibition
glycine
-
8 mM: 99% of activity compared to H2O
HgCl2
-
1 mM: strong inhibition
iodoacetate
-
2 mM: strong inhibition, protected partially, about 30%, by preincubation, 15 min, with 20 mM 2-mercaptoethanol
L-alanine
L-asparagine
-
strong inhibition, 8 mM: 76% of activity compared to H2O
L-aspartic acid
-
strong inhibition, 8 mM: 53% of activity compared to H2O
L-Citric acid
-
8 mM: 95% of activity compared to H2O
L-Glutamic acid
-
strong inhibition, 0.7 mM: 86%, 3.3 mM: 58%, 8 mM: 56%, 53.3 mM: 25% of activity compared to H2O
L-glutamine
-
strong inhibition, 8 mM: 75% of activity compared to H2O
L-leucine
-
partial inhibition, 8 mM: 89% of activity compared to H2O
L-serine
-
partial inhibition, 8 mM: 95% of activity compared to H2O
Maleic acid
-
8 mM: 98% of activity compared to H2O
oxaloacetic acid
-
strong inhibition, 8 mM: 59% of activity compared to H2O
succinic acid
-
strong inhibition, 8 mM: 77% of activity compared to H2O
additional information
-