3.5.1.59: N-carbamoylsarcosine amidase

This is an abbreviated version!
For detailed information about N-carbamoylsarcosine amidase, go to the full flat file.

Word Map on EC 3.5.1.59

Reaction

N-carbamoylsarcosine
+
H2O
=
sarcosine
+
CO2
+
NH3

Synonyms

amidase, carbamoylsarcosine, CSHase, N-carbamoylsarcosine amidohydrolase, N-carbamoylsarcosine amidase Ta0454

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.59 N-carbamoylsarcosine amidase

Crystallization

Crystallization on EC 3.5.1.59 - N-carbamoylsarcosine amidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
structure solved and refined at a resolution of 2.35 A. The enzyme shares a similar fold and a highly conserved C-D-K-catalytic triad, Cys123, Asp9, and Lys90 with the structures of three cysteine hydrolases (PDB codes: 1NBA, 1IM5, and 2HOR). Molecular dynamics simulations of Ta0454/N-carbamoylsarcosine and Ta0454/pyrazinamide complexes are performed to determine the structural basis of the substrate binding pattern for each ligand. The predicted binding free energies suggest that Ta0454 is selective for N-carbamoylsarcosine over pyrazinamide, and zinc ions play an important role in the favorable substrate bound states