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3.5.1.5: urease

This is an abbreviated version!
For detailed information about urease, go to the full flat file.

Word Map on EC 3.5.1.5

Reaction

Urea
+
H2O
=
CO2
 + 2 NH3

Synonyms

acid urease, Arthritogenic cationic 19 kDa antigen, BPU, canatoxin, embryo-specific soybean urease, Eu1, Eu4, HPU, jack bean urease, JBU, JBURE-II, More, PMU, urea amido hydrolase, Urea amidohydrolase, urease, urease JBURE-IIb, UreC

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.5 urease

Storage Stability

Storage Stability on EC 3.5.1.5 - urease

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-4°C, purified native enzyme retains about 14.1% after 2 months, half-life is 22.4 days
-
20°C, 50 mM phosphate buffer, pH 7.5, 50% glycerol, 1 mM EDTA, 5 mM 2-mercaptoethanol, about 30% loss of activity after 2 months
-
4°C, 70 days, soluble enzyme loses 90% of its activity, alkylamine-immobilized enzyme loses 15% of the initial activity, the arylamine-immobilized enzyme loses 17% of its activity
-
4°C, PBS buffer, pH 7.2, enzyme immobilized by cross linking using glutaraldehyde, about 50% loss of activity after 60 days
-
4°C, PBS buffer, pH 7.2, soluble enzyme loses about 40% of its activity after 10 days
-
4°C, sodium phosphate buffer, pH 7.2, 1% bovine serum albumin, stable for 20 days
-