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3.5.1.5: urease

This is an abbreviated version!
For detailed information about urease, go to the full flat file.

Word Map on EC 3.5.1.5

Reaction

Urea
+
H2O
=
CO2
 + 2 NH3

Synonyms

acid urease, Arthritogenic cationic 19 kDa antigen, BPU, canatoxin, embryo-specific soybean urease, Eu1, Eu4, HPU, jack bean urease, JBU, JBURE-II, More, PMU, urea amido hydrolase, Urea amidohydrolase, urease, urease JBURE-IIb, UreC

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.5 urease

Molecular Weight

Molecular Weight on EC 3.5.1.5 - urease

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
11000
11100
11129
x * 61054 + x * 11377 + x * 11129, calculated from sequence
11377
x * 61054 + x * 11377 + x * 11129, calculated from sequence
113900
-
gamma subunit, method not specified
11700
-
beta subunit, method not specified
12000
125000
-
gel filtration
130000
-
sucrose density gradient centrifugation
130000 - 135000
gel filtration
135000
-
non-denaturing PAGE
13900
-
alpha4beta4gamma4, 4 * 72400 + 4 * 20400, + 4 * 13900, SDS-PAGE
14000
140000
-
gel filtration
14200
1 * 67000, alpha-subunit, + 1 * 14200, beta-subunit, + 1 * 11100, gamma-subunit, SDS-PAGE
15000
-
alpha2beta2gamma2, 2 * 64000 + 2 * 15500 + 2 * 15000, SDS-PAGE
150000
-
gel filtration
15500
157000
nondenaturing PAGE
172000
-
x * 172000, SDS-PAGE
174000
-
x * 174000 + x * 179000, SDS-PAGE
174500
-
native PAGE
175000
-
gel filtration
179000
180000
-
sucrose density gradient centrifugation
185000
190000
-
gel filtration
200000
Streptococcus mitior
-
gel filtration
20400
21000
215000
-
gel filtration
220000
224000
-
gel filtration
230000
-
gel filtration
232000
-
gel filtration
242000
-
gel filtration
260000
bacterium strain SL100
-
gel filtration
26500
-
beta subunit, method not specified
269000
-
gel filtration
270000
-
non-denaturing PAGE
280000
-
urease 1, non-denaturing PAGE
29000
-
SDS-PAGE, subunits of the purified urease are shown to be stoichiometrically equal, with estimated molecular masses of 63 kDa and 29kDa
29200
-
3 * 29200 + 3 * 64800, SDS-PAGE
30000
-
SDS-PAGE, two major bands at 30 kDa and 60 kDa
30100
-
3 * 30100 + 3 * 64300, SDS-PAGE
30200
-
3 * 30200 + 3 * 69300, SDS-PAGE
30400
-
3 * 30400 + 3 * 64300, SDS-PAGE
31000
-
x * 31000 + x * 66000, SDS-PAGE
32000
-
x * 65000 (UreA) + x * 32000 (UreB), SDS-PAGE
350000
-
gel filtration
38000
-
x * 38000, SDS-PAGE
380000
-
non-denaturing PAGE
410000
-
non-denaturing PAGE
420000
-
non-denaturing PAGE
430000
-
gel filtration
480000
484000
544000
-
gel filtration in presence of 25% NaCl
545000
-
native PAGE
545300
-
hexamer with 12 nickel ions included, method not specified
580000
-
gel filtration
590000
-
hexamer with 12 nickel ions included, sedimentation method
60000
-
SDS-PAGE, two major bands at 30 kDa and 60 kDa
600000
-
gel filtration
60300
-
alpha subunit, method not specified
61000
61054
x * 61054 + x * 11377 + x * 11129, calculated from sequence
61400
-
alpha subunit, method not specified
62000
-
x + 11000 + x * 11000 + x * 62000, SDS-PAGE
63000
-
SDS-PAGE, subunits of the purified urease are shown to be stoichiometrically equal, with estimated molecular masses of 63 kDa and 29kDa
64000
64300
64800
-
3 * 29200 + 3 * 64800, SDS-PAGE
65000
65500
-
4 * 65500, SDS-PAGE
66000
67000
69300
-
3 * 30200 + 3 * 69300, SDS-PAGE
72000
72400
9000
-
alpha2beta4tau4, 2 * 72000 + 4 * 11000 + 4 * 9000, SDS-PAGE
90000
90500
-
2 * 90500, SDS-PAGE
90770
-
alpha subunit of jack bean urease without Ni2+, method not specified
93500
-
x * 93500, SDS-PAGE
95000
-
x * 95000, SDS-PAGE, equilibrium sedimentation in presence of guanidinium chloride and 2-mercaptoethanol