3.5.1.5: urease

0.5 mM, 30°C, pH 6.8, 56.5% inhibition

(2-[[(2-[[2-(hydroxy-kappaO)ethyl]amino-kappaN]ethyl)imino-kappaN]methyl]-4-nitrophenolato-kappaO)(methoxymethanolato-kappaO)zinc

711908

(2-[[(2-[[2-(hydroxy-kappaO)ethyl]amino-kappaN]ethyl)imino-kappaN]methyl]-4-nitrophenolato-kappaO)(nitrato-kappaO)copper

711908

(2E)-3-(1,3-benzodioxol-5-yl)-1-(2-hydroxyphenyl)prop-2-en-1-one

(2R,3S)-2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-1-benzopyran-3,5,7-triol

0.5 mM, 30°C, pH 6.8, 81.5% inhibition

(2S)-2-(dimethylamino)-3-(ethylthio)propan-1-ol

(2S)-2-(dimethylamino)-3-ethoxypropane-1-thiol

(2S)-2-(dimethylamino)-3-mercaptopropan-1-ol

(2S)-2-(dimethylamino)-3-methoxypropane-1-thiol

(aminomethyl)methylphosphinic acid

718558

(aminomethyl)phosphonic acid

(E)-1-((E)-3-phenylallylidene)thiosemicarbazide

(E)-1-(2-chlorobenzylidene)thiosemicarbazide

(E)-1-(3,4,5-trimethoxybenzylidene)thiosemicarbazide

(E)-1-(3-bromobenzylidene)thiosemicarbazide

(E)-1-(3-chlorobenzylidene)thiosemicarbazide

(E)-1-(3-methoxybenzylidene)thiosemicarbazide

(E)-1-(3-nitrobenzylidene)thiosemicarbazide

competitive mechanism of inhibition

(E)-1-(4-(N,N-dimethylamino)benzylidene)thiosemicarbazide

(E)-1-(4-bromobenzylidene)thiosemicarbazide

(E)-1-(4-chlorobenzylidene)thiosemicarbazide

(E)-1-(4-methoxybenzylidene)thiosemicarbazide

(E)-1-(4-nitrobenzylidene)thiosemicarbazide

(E)-2-((E)-but-2-enylidene)hydrazinecarbothioamide

(E)-2-(2,3-dihydroxybenzylidene)hydrazinecarbothioamide

(E)-2-(2-(benzyloxy)benzylidene)hydrazinecarbothioamide

(E)-2-(3-(benzyloxy)-4-methoxybenzylidene)hydrazinecarbothioamide

(E)-2-(4-hydroxy-3,5-dimethoxybenzylidene)hydrazinecarbothioamide

(E)-2-(4-hydroxybenzylidene)hydrazinecarbothioamide

(Hg2)2+

(R)-4-methoxydalbergione

(R)-dalbergiophenol

([[(benzyloxy)carbonyl]amino]methyl)methylphosphinothioic O-acid

1'-(trifluoroacetyl)-1,4'-bipiperidine

50% inhibition at 0.061 mM

1,1-dimethyl-3-[(1S)-1-naphthalen-1-ylethyl]selenourea

1,10-phenanthroline

Rhodobacter capsulatus

5 mM, 67% inhibition

209171

1,3-bis(3-chlorophenyl)thiourea

1,4-Naphthoquinone

1-(2,4-difluorophenyl)-3-phenylthiourea

1-(2,4-dihydroxyphenyl)-2-(4-fluorophenyl)ethanone oxime

1-(2,4-dihydroxyphenyl)-2-(4-hydroxyphenyl)ethanone

0.4 mg/ml, 16% inhibition

1-(2,4-dihydroxyphenyl)-2-(4-methoxyphenyl)ethanone oxime

1-(2,5-dimethoxyphenyl)-3-phenylthiourea

1-(2-chlorophenyl)-3-(3-chlorophenyl)thiourea

1-(2-chloropyridin-3-yl)-3-phenylthiourea

1-(2-hydroxy-4,6-dimethoxyphenyl)-2-(4-methoxyphenyl)ethanone oxime

1-(3,4-dihydroxyphenyl)-2-(4-hydroxyphenyl)ethanone

0.4 mg/ml, 39% inhibition

1-(3,4-dihydroxyphenyl)-2-(4-hydroxyphenyl)ethanone oxime

1-(3,5-dichloropyridin-2-yl)-3-phenylthiourea

1-(3-bromophenyl)-3-phenylthiourea

1-(3-chlorophenyl)-3-(2,3-dichlorophenyl)thiourea

1-(3-chlorophenyl)-3-(2,4-dichlorophenyl)thiourea

1-(3-chlorophenyl)-3-(2,4-dimethylphenyl)thiourea

1-(3-chlorophenyl)-3-(2,5-dimethylphenyl)thiourea

1-(3-chlorophenyl)-3-(2,6-dichlorophenyl)thiourea

1-(3-chlorophenyl)-3-(2,6-dimethylphenyl)thiourea

1-(3-chlorophenyl)-3-(2-chloropyridin-3-yl)thiourea

1-(3-chlorophenyl)-3-(2-methoxyphenyl)thiourea

1-(3-chlorophenyl)-3-(3,4-dichlorophenyl)thiourea

1-(3-chlorophenyl)-3-(3,4-difluorophenyl)thiourea

1-(3-chlorophenyl)-3-(3-methoxyphenyl)thiourea

1-(3-chlorophenyl)-3-(3-methylpyridin-2-yl)thiourea

1-(3-chlorophenyl)-3-(4-chlorophenyl)thiourea

1-(3-chlorophenyl)-3-(4-fluorophenyl)thiourea

1-(3-chlorophenyl)-3-(4-methoxyphenyl)thiourea

1-(3-chlorophenyl)-3-(m-tolyl)thiourea

1-(3-chlorophenyl)-3-(o-tolyl)thiourea

1-(3-chlorophenyl)-3-(p-tolyl)thiourea

1-(3-chlorophenyl)-3-(pyridin-3-yl)thiourea

1-(3-chlorophenyl)-3-(pyridin-3-ylmethyl)thiourea

1-(3-chlorophenyl)-3-(pyridin-4-ylmethyl)thiourea

1-(3-methylpyridin-2-yl)-3-phenylthiourea

1-(4-bromophenyl)-3-phenylthiourea

1-(5-bromo-6-methylpyridin-2-yl)-3-phenylthiourea

1-(5-chloro-2-methylphenyl)-3-(3-chlorophenyl)thiourea

1-(furan-2-ylmethyl)-3-phenylthiourea

1-benzyl-2-ethyl-6,6-dimethyl-1,5,6,7-tetrahydro-4H-benzimidazol-4-one

reversible and competitive inhibitor

1-benzyl-2-ethyl-6-(2,4,6-trimethylphenyl)-1,5,6,7-tetrahydro-4H-benzimidazol-4-one

reversible and competitive inhibitor

1-cyclohexyl-3,3-dimethyl-1-(1-methylethyl)selenourea

1-ethoxy-2-ethyl-6,6-dimethyl-1,5,6,7-tetrahydro-4H-benzimidazol-4-one

reversible and competitive inhibitor

1-ethoxy-2-ethyl-6-(2,4,6-trimethylphenyl)-1,5,6,7-tetrahydro-4H-benzimidazol-4-one

reversible and competitive inhibitor

1-phenyl-3-(pyridin-2-yl)thiourea

1-phenyl-3-(pyridin-3-yl)thiourea

1-phenyl-3-(pyridin-3-ylmethyl)thiourea

1-phenyl-3-(pyridin-4-yl)thiourea

1-phenyl-3-(pyridin-4-ylmethyl)thiourea

10-hydroxyaloin A

0.5 mM, 30°C, pH 6.8, 46.9% inhibition

12-hydroxytetradecanoic acid

48.47% inhibition at 100 ng/ml

2',4',5',7-tetramethoxyisoflavone

2,2'-bi-1,3,4-oxadiazole-5,5'(4H,4'H)-dithione

2,2'-bi-1,3,4-thiadiazole-5,5'(4H,4'H)-dithione

2,2'-thenoin

IC50: 0.18 mM

654956

2,3,4,4'-tetrahydroxydeoxybenzoin

0.4 mg/ml, 51% inhibition

2,3,4-trihydroxy-4'-methoxydeoxybenzoin

0.4 mg/ml, 44% inhibition

2,3-dichloro-N'-(2,3-dichlorobenzoyl)benzohydrazide

2,3-dichloro-N'-(3,5-dichlorobenzoyl)benzohydrazide

2,3-dichloro-N'-(3,5-dimethylbenzoyl)benzohydrazide

2,3-dichloro-N'-(3-chlorobenzoyl)benzohydrazide

2,4,4',6-tetrahydroxy-3-methoxyphenyldeoxybenzoin

0.4 mg/ml, 17% inhibition

2,4,6-trihydroxy-9H-xanthen-9-one

712598

2,4,6-trimethyl-N-([1-[(2,4,6-trimethylphenyl)sulfonyl]piperidin-4-yl]methyl)benzenesulfonamide

50% inhibition at 0.038 mM

2,4-dichloro-N'-(2,4-dichlorobenzoyl)benzohydrazide

2,4-dichloro-N'-(3,5-dichlorobenzoyl)benzohydrazide

2,4-dihydroxy-4'-methoxydeoxybenzoin

0.4 mg/ml, 14% inhibition

2,4-dihydroxyacetophenone

0.4 mg/ml, 14% inhibition

2,4-diphenyl-2,3-dihydro-1,5-benzothiazepine

2-(1-amino-2-(4-hydroxyphenyl)ethyl)-4-methoxybenzene-1,3,5-triol

2-(1-amino-2-(4-hydroxyphenyl)ethyl)benzene-1,3,5-triol

2-(4-fluorophenyl)-1-(2,3,4-trihydroxyphenyl)ethanone oxime

2-(4-hydroxyphenyl)-1-(2,3,4-trihydroxyphenyl)ethanone oxime

2-(4-hydroxyphenyl)-1-(2,4,6-trihydroxy-3-methoxyphenyl)ethanone oxime

2-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)ethanone oxime

2-(4-methoxyphenyl)-1-(2,3,4-trimethoxyphenyl)ethanamine

2-(4-methylphenyl)-4-phenyl-2,3-dihydro-1,5-benzothiazepine

2-(4-nitrophenyl)-4-phenyl-2,3-dihydro-1,5-benzothiazepine

2-chloro-N'-(3-chlorobenzoyl)-4-methylbenzohydrazide

2-ethyl-1-(2-phenylethyl)-6-(2,4,6-trimethylphenyl)-1,5,6,7-tetrahydro-4H-benzimidazol-4-one

reversible and competitive inhibitor

2-ethyl-1-(4-methoxycyclohexyl)-6,6-dimethyl-1,5,6,7-tetrahydro-4H-benzimidazol-4-one

reversible and competitive inhibitor

2-ethyl-1-(4-methoxyphenyl)-6-(2,4,6-trimethylphenyl)-1,5,6,7-tetrahydro-4H-benzimidazol-4-one

reversible and competitive inhibitor

2-ethyl-6-[2-(ethylsulfanyl)propyl]-1-(4-methoxyphenyl)-1,5,6,7-tetrahydro-4H-benzimidazol-4-one

reversible and competitive inhibitor

2-hydroxy-1-phenylethanone

IC50: 0.47 mM

654956

2-iodo-N'-(3-methylbenzoyl)benzohydrazide

2-mercaptoethanol

2-thioxo-5-(2,3,4-trihydroxybenzylidene)dihydro-4,6(1H,5H)-pyrimidinedione

2-[(1E)-N-(allyloxy)butanimidoyl]-5,5-dimethylcyclohexane-1,3-dione

chelator of the nickel atom in enzyme metallocenter

2-[(1E)-N-ethoxybutanimidoyl]-5,5-dimethylcyclohexane-1,3-dione

chelator of the nickel atom in enzyme metallocenter

2-[(2S,4R)-2-(4-fluorophenyl)-2,3,4,5-tetrahydro-1,5-benzothiazepin-4-yl]phenol

2-[(2S,4R)-2-phenyl-2,3,4,5-tetrahydro-1,5-benzothiazepin-4-yl]phenol

2-[2-(1,3-benzodioxol-5-yl)-2,3-dihydro-1,5-benzothiazepin-4-yl]phenol

2-[2-(3,4-dimethoxyphenyl)ethyl]phenol

2-[4-(2-methylpropyl)phenyl]-N-[(2,4,6-trimethylphenyl)carbamothioyl]propanamide

2-[4-(2-methylpropyl)phenyl]-N-[(3-nitrophenyl)carbamothioyl]propanamide

2-[4-(2-methylpropyl)phenyl]-N-[(4-sulfamoylphenyl)carbamothioyl]propanamide

3,3'-(1H-indol-3-ylmethanediyl)bis(4-hydroxy-2H-chromen-2-one)

3,3'-(1H-pyrrol-2-ylmethanediyl)bis(4-hydroxy-2H-chromen-2-one)

3,3'-(2-bromobenzylidene)-bis-(4-hydroxycoumarin)

3,3'-(2-chlorobenzylidene)-bis-(4-hydroxycoumarin)

3,3'-(2-methoxybenzylidene)-bis-(4-hydroxycoumarin)

3,3'-(2-phenylpropane-1,1-diyl)bis(4-hydroxy-2H-chromen-2-one)

3,3'-(2-phenylpropylidene)-bis-(4-hydroxycoumarin)

3,3'-(2-pyridyl-methylene)-bis-(4-hydroxycoumarin)

3,3'-(2-pyrolidene)-bis-(4-hydroxycoumarin)

3,3'-(2E)-but-2-ene-1,1-diylbis(4-hydroxy-2H-chromen-2-one)

3,3'-(3-chlorobenzylidene)-bis-(4-hydroxycoumarin)

3,3'-(3-cinnamylidene)-bis-(4-hydroxycoumarin)

3,3'-(3-hydroxybenzylidene)-bis-(4-hydroxycoumarin)

3,3'-(3-indolidene)-bis-(4-hydroxycoumarin)

3,3'-(3-methoxybenzylidene)-bis-(4-hydroxycoumarin)

3,3'-(3-phenylpropane-1,1-diyl)bis(4-hydroxy-2H-chromen-2-one)

3,3'-(4-hydroxybenzylidene)-bis-(4-hydroxycoumarin)

3,3'-(4-methoxybenzylidene)-bis-(4-hydroxycoumarin)

3,3'-(4-nitrobenzylidene)-bis-(4-hydroxycoumarin)

3,3'-(crotonalidene)-bis-(4-hydroxycoumarin)

3,3'-(dihydrocinnamylidene)-bis-(4-hydroxycoumarin)

3,3'-(naphthalen-1-ylmethanediyl)bis(4-hydroxy-2H-chromen-2-one)

3,3'-(pyridin-3-ylmethanediyl)bis(4-hydroxy-2H-chromen-2-one)

3,3'-(pyridin-4-ylmethanediyl)bis(4-hydroxy-2H-chromen-2-one)

3,3'-butane-1,1-diylbis(4-hydroxy-2H-chromen-2-one)

3,3'-ethane-1,1-diylbis(4-hydroxy-2H-chromen-2-one)

3,3'-ethylidenebis-(4-hydroxycoumarin)

3,3'-hexane-1,1-diylbis(4-hydroxy-2H-chromen-2-one)

3,3'-methanediylbis(4-hydroxy-2H-chromen-2-one)

3,3'-methylenebis-(4-hydroxycoumarin)

3,3'-n-butylidene-bis(4-hydroxycoumarin)

3,3'-n-hexylidene-bis(4-hydroxycoumarin)

3,3'-n-pentylidene-bis(4-hydroxycoumarin)

3,3'-pentane-1,1-diylbis(4-hydroxy-2H-chromen-2-one)

3,3'-[(1E)-1-phenylprop-1-ene-3,3-diyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(2-bromophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(2-chlorophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(2-methoxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(3,4,5-trimethoxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(3,4-dimethoxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(3-aminophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(3-chlorophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(3-ethoxy-4-hydroxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(3-hydroxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(3-methoxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(3-nitrophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(4-hydroxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(4-methoxyphenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[(4-nitrophenyl)methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[[4-(1-methylethyl)phenyl]methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,3'-[[4-(dimethylamino)phenyl]methanediyl]bis(4-hydroxy-2H-chromen-2-one)

3,4,5-trihydroxy-9H-xanthen-9-one

712598

3,4,5-Trihydroxybenzoate

3,4-Dihydroxyacetophenone

0.4 mg/ml, 41% inhibition

3,5-dichloro-N'-(3-chlorobenzoyl)benzohydrazide

3,5-dichloro-N'-(3-methylbenzoyl)benzohydrazide

3,5-dichloro-N'-(4-chlorobenzoyl)benzohydrazide

3,5-dichloro-N'-(4-methoxybenzoyl)benzohydrazide

3,5-dichloro-N'-(4-methylbenzoyl)benzohydrazide

3,5-dimethylphenyl diamidophosphate

3-(4-bromobenzyl)-1,1-dimethylselenourea

3-(4-bromophenyl)-1,1-dimethylthiourea

3-chloro-N'-(3-chlorobenzoyl)benzohydrazide

3-chloro-N'-(3-methylbenzoyl)benzohydrazide

3-chloro-N'-(3-nitrobenzoyl)benzohydrazide

3-chloro-N'-(4-methylbenzoyl)benzohydrazide

3-dodecyl-1,1-dimethylthiourea

3-hydroxytetradecanoic acid

49.20% inhibition at 100 ng/ml

3-methyl-N'-(3-methylbenzoyl)benzohydrazide

3-methyl-N-[[1-(3-methylbut-2-en-1-yl)piperidin-4-yl]methyl]but-2-en-1-amine

50% inhibition at 0.063 mM

3-phenoxy-N-[[1-(3-phenoxypropyl)piperidin-4-yl]methyl]propan-1-amine

50% inhibition at 0.043 mM

3-[(2-acetylanilino)methylidene]-8-methyl-2H-pyrido[1,2-a]-pyrimidine-2,4-dione

54% inhibition at 0.01 mM

3-[(2-fluoroanilino)methylidene]-8-methyl-2H-pyrido[1,2-a]-pyrimidine-2,4-dione

44% inhibition at 0.01 mM

3-[(3-acetylanilino)methylidene]-8-methyl-2H-pyrido[1,2-a]-pyrimidine-2,4-dione

28% inhibition at 0.01 mM

3-[(3-fluoroanilino)methylidene]-8-methyl-2H-pyrido[1,2-a]-pyrimidine-2,4-dione

3% inhibition at 0.01 mM

3-[(4-acetylanilino)methylidene]-8-methyl-2H-pyrido[1,2-a]pyrimidine-2,4-dione

5% inhibition at 0.01 mM

3-[(4-fluoroanilino)methylidene]-8-methyl-2H-pyrido[1,2-a]pyrimidine-2,4-dione

21% inhibition at 0.01 mM

3-[(4-[[5-(2-bromophenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)methyl]-4-hydroxy-2H-1-benzopyran-2-one

3-[(4-[[5-(2-chlorophenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)methyl]-4-hydroxy-2H-1-benzopyran-2-one

3-[(4-[[5-(2-fluorophenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)methyl]-4-hydroxy-2H-1-benzopyran-2-one

3-[(4-[[5-(3-bromophenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)methyl]-4-hydroxy-2H-1-benzopyran-2-one

3-[(4-[[5-(3-chlorophenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)methyl]-4-hydroxy-2H-1-benzopyran-2-one

3-[(4-[[5-(3-fluorophenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)methyl]-4-hydroxy-2H-1-benzopyran-2-one

3-[(4-[[5-(4-bromophenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)methyl]-4-hydroxy-2H-1-benzopyran-2-one

3-[(4-[[5-(4-chlorophenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)methyl]-4-hydroxy-2H-1-benzopyran-2-one

3-[(4-[[5-(4-fluorophenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)methyl]-4-hydroxy-2H-1-benzopyran-2-one

3-[(mesitylamino)methylidene]-8-methyl-2H-pyrido[1,2-a]pyrimidine-2,4-dione

4'-O-methylalpinumisoflavone

4'-O-methylderrone

4-(1-amino-2-(4-fluorophenyl)ethyl)benzene-1,2,3-triol

4-(1-amino-2-(4-fluorophenyl)ethyl)benzene-1,3-diol

4-(1-amino-2-(4-hydroxyphenyl)ethyl)benzene-1,2,3-triol

4-(1-amino-2-(4-hydroxyphenyl)ethyl)benzene-1,2-diol

4-(1-amino-2-(4-methoxyphenyl)ethyl)benzene-1,3-diol

4-(2,3-dimethylphenyl)-5-phenyl-2,4-dihydro-3H-1,2,4-triazole-3-thione

4-(2,4-dichlorophenyl)-6-(9-methyl-9H-carbazol-3-yl)-pyrimidin-2-amine

4-(2,4-difluorophenyl)-6-(9-methyl-9H-carbazol-3-yl)-pyrimidin-2-amine

4-(2,4-dimethylphenyl)-5-(3-nitrophenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione

4-(2,5-dichlorophenyl)-6-(9-methyl-9H-carbazol-3-yl)-pyrimidin-2-amine

4-(3,4-dichlorophenyl)-6-(9-methyl-9H-carbazol-3-yl)-pyrimidin-2-amine

4-(3,4-difluorophenyl)-6-(9-methyl-9H-carbazol-3-yl)-pyrimidin-2-amine

4-(3,4-dimethoxyphenyl)-6-(9-methyl-9H-carbazol-3-yl)-pyrimidin-2-amine

4-(3-aminophenyl)-6-(9-methyl-9H-carbazol-3-yl)-pyrimidin-2-amine

4-(4-benzoyl-5-thioxo-4,5-dihydro-1,3,4-thiadiazol-2-yl)phenyl benzoate

4-(4-bromophenyl)-5-[hydroxy(phenyl)methyl]-2,4-dihydro-3H-1,2,4-triazole-3-thione

4-(4-bromophenyl)-6-(9-methyl-9H-carbazol-3-yl)-pyrimidin-2-amine

4-(4-chlorophenyl)-5-[hydroxy(phenyl)methyl]-2,4-dihydro-3H-1,2,4-triazole-3-thione

4-(4-chlorophenyl)-6-(9-methyl-9H-carbazol-3-yl)-pyrimidin-2-amine

4-(4-fluorophenyl)-6-(9-methyl-9H-carbazol-3-yl)-pyrimidin-2-amine

4-(4-methoxyphenyl)-6-(9-methyl-9H-carbazol-3-yl)-pyrimidin-2-amine

4-(9-methyl-9H-carbazol-3-yl)-6-(2,4,6-trimethoxyphenyl)-pyrimidin-2-amine

4-(9-methyl-9H-carbazol-3-yl)-6-(4-nitrophenyl)-pyrimidin-2-amine

4-(9-methyl-9H-carbazol-3-yl)-6-(pyridin-2-yl)-pyrimidin-2-amine

4-(9-methyl-9H-carbazol-3-yl)-6-(thiophen-2-yl)-pyrimidin-2-amine

4-(9-methyl-9H-carbazol-3-yl)-6-phenylpyrimidin-2-amine

4-(benzyloxy)phenyl diamidophosphate

4-(methoxymethyl)-5-methylbenzene-1,3-diol

0.5 mM, 30°C, pH 6.8, 91.5% inhibition

4-(p-hydroxyphenethyl)pyrogallol

0.4 mg/ml, 94.5% inhibition

4-amino-5-(4-hydroxyphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione

4-amino-5-pyridin-4-yl-2,4-dihydro-3H-1,2,4-triazole-3-thione

4-bromo-N'-[[8-methyl-2,4-dioxo-2H-pyrido[1,2-a]pyrimidin-3(4H)-ylidene]methyl]benzohydrazide

23% inhibition at 0.01 mM

4-bromophenylboronic acid

4-butylbenzene-1,2,3-triol

4-butylbenzene-1,2-diol

4-chloro-N'-[[8-methyl-2,4-dioxo-2H-pyrido[1,2-a]pyrimidin-3(4H)-ylidene]methyl]benzohydrazide

14% inhibition at 0.01 mM

4-decylbenzene-1,2,3-triol

4-dodecylbenzene-1,2,3-triol

4-ethylbenzene-1,2,3-triol

4-ethylbenzene-1,2-diol

4-hexylbenzene-1,2,3-triol

4-hexylbenzene-1,2-diol

4-hydroxy-3-[(2E)-1-(4-hydroxy-2-oxo-4a,8a-dihydro-2H-chromen-3-yl)-3-phenylprop-2-en-1-yl]-2H-chromen-2-one

4-hydroxy-3-[(2E)-1-(4-hydroxy-2-oxo-4a,8a-dihydro-2H-chromen-3-yl)but-2-en-1-yl]-2H-chromen-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)(4-[[5-(2-methoxyphenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)methyl]-2H-1-benzopyran-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)(4-[[5-(2-methylphenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)methyl]-2H-1-benzopyran-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)(4-[[5-(3-methoxyphenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)methyl]-2H-1-benzopyran-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)(4-[[5-(3-methylphenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)methyl]-2H-1-benzopyran-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)(4-[[5-(3-nitrophenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)methyl]-2H-1-benzopyran-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)(4-[[5-(4-methoxyphenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)methyl]-2H-1-benzopyran-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)(4-[[5-(4-methylphenyl)-1,3,4-thiadiazol-2-yl]amino]phenyl)methyl]-2H-1-benzopyran-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)[4-([5-[4-(trifluoromethyl)phenyl]-1,3,4-thiadiazol-2-yl]amino)phenyl]methyl]-2H-1-benzopyran-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-4a,8a-dihydro-2H-chromen-3-yl)(1H-indol-3-yl)methyl]-2H-chromen-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-4a,8a-dihydro-2H-chromen-3-yl)(1H-pyrrol-3-yl)methyl]-2H-chromen-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-4a,8a-dihydro-2H-chromen-3-yl)(3-methoxyphenyl)methyl]-2H-chromen-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-4a,8a-dihydro-2H-chromen-3-yl)(pyridin-4-yl)methyl]-2H-chromen-2-one

4-hydroxy-3-[(4-hydroxy-2-oxo-4a,8a-dihydro-2H-chromen-3-yl)methyl]-2H-chromen-2-one

4-hydroxy-3-[1-(4-hydroxy-2-oxo-4a,8a-dihydro-2H-chromen-3-yl)ethyl]-2H-chromen-2-one

4-hydroxy-3-[1-(4-hydroxy-2-oxo-4a,8a-dihydro-2H-chromen-3-yl)pentyl]-2H-chromen-2-one

4-hydroxy-N'-[[8-methyl-2,4-dioxo-2H-pyrido[1,2-a]pyrimidin-3(4H)-ylidene]methyl]benzohydrazide

22% inhibition at 0.01 mM

4-methyl-N-([1-[(4-methylphenyl)sulfonyl]piperidin-4-yl]methyl)benzenesulfonamide

50% inhibition at 0.042 mM

4-nitro-N'-[[8-methyl-2,4-dioxo-2H-pyrido[1,2-a]pyrimidin-3(4H)-ylidene]methyl] benzohydrazide

60% inhibition at 0.01 mM

4-nitro-N-([1-[(4-nitrophenyl)sulfonyl]piperidin-4-yl]methyl)benzenesulfonamide

50% inhibition at 0.032 mM

4-nitrophenyl diamidophosphate

4-octylbenzene-1,2,3-triol

4-octylbenzene-1,2-diol

4-tetradecylbenzene-1,2,3-triol

4-[2-(3,4-diethoxyphenyl)ethyl]benzene-1,2,3-triol

4-[2-(3,4-dimethoxyphenyl)ethyl]benzene-1,2,3-triol

4-[2-(3,4-dimethoxyphenyl)ethyl]benzene-1,2-diol

4-[2-(3,4-dimethoxyphenyl)ethyl]phenol

4-[2-(3-bromophenyl)ethyl]benzene-1,2,3-triol

4-[2-(3-bromophenyl)ethyl]benzene-1,2-diol

4-[2-(3-chlorophenyl)ethyl]-1,2-dimethoxybenzene

4-[2-(3-chlorophenyl)ethyl]benzene-1,2,3-triol

4-[2-(3-chlorophenyl)ethyl]benzene-1,2-diol

4-[2-(4-bromophenyl)ethyl]benzene-1,2,3-triol

4-[2-(4-chlorophenyl)ethyl]-1,2-dimethoxybenzene

4-[2-(4-chlorophenyl)ethyl]benzene-1,2,3-triol

4-[2-(4-chlorophenyl)ethyl]benzene-1,2-diol

4-[2-(4-fluorophenyl)ethyl]benzene-1,2,3-triol

4-[2-(4-fluorophenyl)ethyl]benzene-1,2-diol

4-[2-(4-hydroxyphenyl)ethyl]benzene-1,2,3-triol

4-[2-(4-hydroxyphenyl)ethyl]benzene-1,2-diol

5-(1,4-dihydro-9-anthracenylmethylene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(1-naphthyl)-1,3,4-oxadiazole-2(3H)-thione

5-(1H-indol-3-ylmethylene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(2,4-dihydroxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(2-aminobenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(2-bromo-4,5-dimethoxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(2-chlorophenyl)-N-(2,6-dimethylphenyl)-1,3,4-thiadiazol-2-amine

5-(2-hydroxy-3-methoxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(2-hydroxy-5-methoxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(2-methylbenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(2-thienylmethylene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(3,4-dihydroxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(3,4-dimethoxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(3,5-dibromo-4-hydroxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(3,5-dichloro-2-hydroxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(3-chlorophenyl)-4-(2,4-dimethylphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione

5-(3-chlorophenyl)-4-(2,6-dimethylphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione

5-(3-hydroxy-4-methoxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(3-hydroxyphenyl)-1,3,4-oxadiazole-2(3H)-thione

5-(4-bromo-2,5-dimethoxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(4-chlorophenyl)-4-(2,4-dimethylphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione

5-(4-chlorophenyl)-N-(2,3-dimethylphenyl)-1,3,4-thiadiazol-2-amine

5-(4-chlorophenyl)-N-(2,6-dimethylphenyl)-1,3,4-thiadiazol-2-amine

5-(4-ethoxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(4-hydroxy-3,5-dimethoxybenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(4-hydroxy-3-iodo-5-methoxybenzylidene)-2-thioxodihydro-4,6(1H,5H) pyrimidinedione

5-(4-hydroxyphenyl)-1,3,4-oxadiazole-2(3H)-thione

5-(4-hydroxyphenyl)-1,3,4-thiadiazole-2(3H)-thione

5-(4-propoxyphenyl)-3-propyl-1,3,4-thiadiazole-2(3H)-thione

5-(4-pyridinylmethylene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-(4-[[4,6-dioxo-2-thioxotetrahydro-5(2H)pyrimidinylidene]methyl]-benzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-benzhydryl-1,3,4-oxadiazole-2(3H)-thione

5-benzhydryl-1,3,4-thiadiazole-2(3H)-thione

5-benzyl-1,3,4-oxadiazole-2(3H)-thione

5-cyclohexyl-1,3,4-oxadiazole-2(3H)-thione

5-fluorouridine

Cucurbita pepo

the combination of 0.3 mM zeatin with the protein inhibitor results in the alleviation of their inhibitory effect on the urease activity

752381

5-hydroxy-1,4-naphthoquinone

5-hydroxy-1,4-naphthoquinone, juglone, acts as a strong, time and concentration dependent inactivator of urease. The reactivation of juglone-modified urease shows the participation of reversible and irreversible contribution in the inactivation. In the presence of an excess of DTT, urease inactivated by juglone regains 70% of its activity. The reversible inactivation is attributed to oxidation of the essential urease thiols by reactive oxygen species (ROS) realizing during reduction of juglone to seminaphthoquinone. The irreversible contribution in the inhibition is assumed as an arylation of urease thiol groups by juglone

711374

5-phenyl-1,3,4-oxadiazole-2(3H)-thione

5-phenyl-1,3,4-thiadiazole-2(3H)-thione

5-pyridin-4-yl-1,3,4-oxadiazole-2(3H)-thione

5-pyridin-4-yl-1,3,4-thiadiazole-2(3H)-thione

5-[(2-hydroxy-1-naphthyl)methylene]-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-[(5-methyl-2-furyl)methylene]-2-thioxodihydro-4,6(1H,5H)pyrimidinedione

5-[(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)methyl]-1,3,4-oxadiazole-2(3H)-thione

5-[(5-thioxo-4,5-dihydro-1,3,4-thiadiazol-2-yl)methyl]-1,3,4-thiadiazole-2(3H)-thione

5-[(6-bromo-4-chloro-2-oxo-2H-chromen-3-yl)methylene]-2-thioxodihydro 4,6(1H,5H)-pyrimidinedione

5-[(6-methyl-2-pyridinyl)methylene]-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-[3-(5-thioxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)propyl]-1,3,4-oxadiazole-2(3H)-thione

5-[3-(benzyloxy)phenyl]-1,3,4-oxadiazole-2(3H)-thione

5-[4(dimethylamino) benzylidene]-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione

5-[4-(benzyloxy)phenyl]-1,3,4-oxadiazole-2(3H)-thione

5-[4-(methylsulfanyl)benzylidene]-2-thioxodihydro-4,6(1H,5H) pyrimidinedione

5-[4-(tert-butyl-dimethyl-silanyloxy)-phenyl]-3H-[1,3,4]oxadiazole-2-thione

5-[4-(tert-butyl-dimethyl-silanyloxy)-phenyl]-3H-[1,3,4]thiadiazole-2-thione

5-[hydroxy(phenyl)methyl]-4-(4-methylphenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione

5-[hydroxy(phenyl)methyl]-4-(4-nitrophenyl)-2,4-dihydro-3H-1,2,4-triazole-3-thione

6'-O-coumaroylaloesin

0.5 mM, 30°C, pH 6.8, 91.3% inhibition

6-(1-hydroxy-2-(4-methoxyphenyl)ethyl)-2,3-dimethoxyphenol

0.4 mg/ml, 42% inhibition

6-hydroxytetradecanoic acid

59.01% inhibition at 100 ng/ml

6a,12a-dehydroxydegueline

7'-hydroxy-2,2'-dioxo-2H,2'H-6,8'-bichromen-7-yl alpha-L-mannopyranoside

7'-hydroxy-2,2'-dioxo-3'-[(2-oxo-2H-chromen-7-yl)oxy]-2H,2'H-8,8'-bichromen-7-yl alpha-D-glucopyranoside

7'-methoxy-2,2'-dioxo-3'-[(2-oxo-2H-chromen-7-yl)oxy]-2H,2'H-8,8'-bichromen-7-yl alpha-D-glucopyranoside

7,8,4'-trihydroxyisoflavone

0.4 mg/ml, 81.7% inhibition

7-hydroxy-2,2'-dioxo-2H,2'H-6,8'-bichromen-7'-yl alpha-L-mannopyranoside

7-hydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one

7-hydroxy-4-methoxy-5-methyl-2H-1-benzopyran-2-one

7-hydroxytetradecanoic acid

53.43% inhibition at 100 ng/ml

7-methoxy-6'-O-coumaroylaloesin

0.5 mM, 30°C, pH 6.8, 93.5% inhibition

7-O-methylcuneantin

7-[(7-methoxy-2-oxo-2H-chromen-3-yl)oxy]-2-oxo-2H-chromen-6-yl alpha-D-glucopyranoside

8-hydroxy-3-[(6-hydroxy-2-oxo-2H-chromen-7-yl)oxy]-2-oxo-2H-chromen-7-yl beta-D-glucopyranoside

8-methyl-2H-pyrido[1,2-a]pyrimidine-2,4(3H)-dione

26% inhibition at 0.01 mM

8-methyl-3-[(2-sulfanylanilino)methylidene]-2H-pyrido[1,2-a]pyrimidine-2,4-dione

73% inhibition at 0.01 mM

8-methyl-3-[[(4-methyl-2-pyridinyl)amino]methylidene]-2Hpyrido[1,2-]pyrimidine-2,4-dione

20% inhibition at 0.01 mM

8-methyl-3-[[(5-methyl-2-pyridinyl)amino] methylidene]-2H-pyrido[1,2-]pyrimidine-2,4-dione

48% inhibition at 0.01 mM

8-methyl-3-[[(5-nitro-2-pyridinyl)amino] methylidene]-2H-pyrido[1,2-a]pyrimidine-2,4-dione

36% inhibition at 0.01 mM

9-hydroxytetradecanoic acid

52.97% inhibition at 100 ng/ml

Acetohydroxamate

Acetohydroxamic acid

acetol

IC50: 2.9 mM

Ag+

Allicin

from garlic, inhibits the biofilm formation and urease activity in vitro in both prelysed and intact cells. A higher concentration of allicin is needed to inhibit the established biofilms

733886

aloe emodin

0.5 mM, 30°C, pH 6.8, 83.5% inhibition

aloe emodin 11-O-alpha-D-rhamnopyranoside

0.5 mM, 30°C, pH 6.8, 53.6% inhibition

aloinoside B

0.5 mM, 30°C, pH 6.8, 45.7% inhibition

alpha-Amanitin

Cucurbita pepo

the combination of 0.3 mM zeatin with the protein inhibitor results in the alleviation of their inhibitory effect on the urease activity

752381

alpinumisoflavone

aminomethyl(P-methyl)phosphinic acid

atalantoflavone

avicularin

Ba2+

beta-(o-methoxyphenyl)-gamma,gamma-bis(8-quinolinoxy)germa-gamma-lactone

Proteus mirabilis

inhibition occurrs in a noncompetitive and concentration-dependent manner. Highly selective in inhibiting the growth of Proteus mirabilis at moderate concentrations. Potential to be developed as antimicrobial agents against Proteus mirabilis infection

684843

beta-(o-methylphenyl)-gamma,gamma-bis(8-quinolinoxy)germa-gamma-lactone

Boric acid

butylboronic acid

Ca2+

Calopogonium isoflavone A

catechol

Cd2+

chloro(2-[[(2-[[2-(hydroxy-kappaO)ethyl]amino-kappaN]ethyl)imino-kappaN]methyl]-4-nitrophenolato-kappaO)copper

711908

citrate

P41022; P41021; P41020

binding of the ligand to the active site involves stabilizing interactions, such as a carboxylate group that binds the nickel ions at the active site and several hydrogen bonds with the surrounding residues

citrate buffer

Co2+

copper(II) complex [CuClL1]*CH3OH

L2 is the deprotonated form of 4-bromo-N'-(2-hydroxy-5-methoxybenzylidene)benzohydrazide

copper(II) complex [CuClL2]*CH3OH

L3 is the deprotonated form of N'-(2-hydroxy-5-methoxybenzylidene)-3-methylbenzohydrazide

copper(II) complex [CuL1(NCS)]

L1 is the deprotonated form of N'-(2-hydroxybenzylidene)-3-methylbenzohydrazide. NCS is N-chlorsuccinimide

copper(II) complex [CuL3(NCS)]*CH3OH

L4 is the deprotonated form of 2-chloro-N'-(2-hydroxy-5-methoxybenzylidene)benzohydrazide

copper(II) complex [CuL4(NCS)]*0.4H2O

L5 is the dianionic form of N'-(2-hydroxybenzylidene)-3-methylbenzohydrazide

copper(II) complex [CuL5(bipy)]

bipy is 2,2'-bipyridine

cordycepin

Cucurbita pepo

the combination of 0.3 mM zeatin with the protein inhibitor results in the alleviation of their inhibitory effect on the urease activity

752381

Cu2+

7 entries

cyanide

Rhodobacter capsulatus

1.0 mM, 32% inhibition

209171

dehydroascorbic acid

inhibitory action of dehydroascorbic acid is revealed in the presence of Fe3+ ions and found to be primarily mediated by H2O2. The resulting inhibition by dehydroascorbic acid -Fe3+ consists of enzyme thiol oxidation and its effectiveness grew with increasing pH

EDTA

emodin

0.5 mM, 30°C, pH 6.8, 46.9% inhibition

epigallocatechin

Klebsiella aerogenes

P18314

strong time-dependent inactivation of urease that is not due to their oxygen sensitivity. Rather, the compound appears to inactivate urease by reacting with a specific Cys residue located on the flexible loop. Substitution of this cysteine by alanine in the C319A variant increases the urease resistance

754249

ethanol

Providencia rettgeri

A0A1U8X5K7

50% inhibition at 30% v/v

754697

ethyl 4-(3-benzoylthioureido) benzoate

ethyl 4-[3-(2,4-dichlorobenzoyl)thioureido]benzoate

ethyl 4-[3-(2-bromobenzoyl)thioureido]benzoate

ethyl 4-[3-(2-fluorobenzoyl)thioureido]benzoate

ethyl 4-[3-(3,4-dimethoxybenzoyl)thioureido]benzoate

ethyl 4-[3-(3-chlorobenzoyl)thioureido]benzoate

ethyl 4-[3-(3-methoxybenzoyl)thioureido]benzoate

ethyl 4-[3-(3-methylbenzoyl)thioureido]benzoate

ethyl 4-[3-(4-methoxybenzoyl)thioureido]benzoate

ethyl 4-[3-(4-methylbenzoyl)thioureido]benzoate

F-

Fe2+

Fe3+

feralolide

0.5 mM, 30°C, pH 6.8, 88.7% inhibition

feroxidin

0.5 mM, 30°C, pH 6.8, 91.3% inhibition

fluoride

furoin

IC50: 0.36 mM

gallacetophenone

0.4 mg/ml, 38% inhibition

guaijaverin

Hg2+

Hpn protein

synthesis and purifcation of the 7 kDa protein, the synthetic nickel-binding histidine-rich protein designated Hpn is capable of abrogating urease activity of live Heicobacter pylori in liquid cultures due to inhibition of nickel uptake into cells

hydroxamic acid

hydroxylamine

Hydroxyurea

hydroxy[3-(5-[4-[(4-hydroxy-2-oxo-2H-1-benzopyran-3-yl)(4-hydroxy-2-oxo-3,4-dihydro-2H-1-benzopyran-3-yl)methyl]anilino]-1,3,4-thiadiazol-2-yl)phenyl]oxoammonium

iodoacetamide

phosphate protects wild-type enzyme from inactivation, does not affect inactivation of C319S urease

isoquercitrin

K+

L-ascorbic acid

in an unbuffered system L-ascorbic acid inactivates urease in a biphasic manner by denaturation brought about by ascorbic acid-lowered pH. In a buffered system neither ascorbic acid nor dehydroascorbic acid themselves are inhibitors of urease

712605

L-aspartyl-4-hydroxamate

L-glutamine

Zea mays

50 mM, 14% inhibition

209153

L-glutamyl-5-hydroxamate

Zea mays

50 mM, 34% inhibition

L-Gly hydroxamate

lyciumaside

0.5 mM, 30°C, pH 6.8, 83.5% inhibition

mercaptoethylamine

inactivates enzyme irreversibly

712722

methyl (7E)-7-[4,4-dimethyl-3-(3-methylisoxazol-5-yl)-2,6-dioxocyclohexyl]-7-(ethoxyimino)heptanoate

chelator of the nickel atom in enzyme metallocenter

methyl 2-amino-4,5,6,7,8,9-hexahydrocycloocta[b]thiophene-3-carboxylate

methyl 2-amino-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate

methyl 2-amino-4-methylthiophene-3-carboxylate

methyl 2-amino-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxylate

methyl 2-amino-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carboxylate

methyl 2-[(methoxycarbonyl)amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate

methyl 2-[bis(chlorocarbonyl)amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate

methyl 2-[[(chloromethoxy)carbonyl]amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate

methyl 2-[[(chloromethoxy)carbonyl]amino]-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxylate

methyl 2-[[(dichloromethoxy)carbonyl]amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate

methyl 2-[[(methylsulfanyl)carbonothioyl]amino]-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate

methyl 3-(4-[[(3-methoxy-3-oxopropyl)amino]methyl]piperidin-1-yl)propanoate

50% inhibition at 0.019 mM

methyl 5-[(1E)-N-(allyloxy)butanimidoyl]-2,2-dimethyl-4,6-dioxocyclohexanecarboxylate

chelator of the nickel atom in enzyme metallocenter

methyl 5-[(1E)-N-ethoxybutanimidoyl]-2,2-dimethyl-4,6-dioxocyclohexanecarboxylate

chelator of the nickel atom in enzyme metallocenter

methyl 5-[(1E)-N-ethoxyethanimidoyl]-2,2-dimethyl-4,6-dioxocyclohexanecarboxylate

chelator of the nickel atom in enzyme metallocenter

methyl[(methylamino)methyl]phosphinic acid

Mg2+

Mn2+

N'-(2,3-dichlorobenzoyl)-3,4,5-trimethoxybenzohydrazide

N'-(2,4-dichlorobenzoyl)pyridine-3-carbohydrazide

N'-(2-bromobenzylidene)-2-(4-nitrophenoxy)acetohydrazide

752383

N'-(2-ethoxybenzoyl)pyridine-3-carbohydrazide

N'-(3-chlorobenzoyl)pyridine-3-carbohydrazide

N'-(3-methylbenzoyl)pyridine-3-carbohydrazide

N'-(4-chlorobenzoyl)pyridine-3-carbohydrazide

N'-(4-methoxybenzoyl)-3,5-dimethylbenzohydrazide

N'-(4-methylbenzoyl)pyridine-3-carbohydrazide

N'-(4-nitrobenzylidene)-2-(4-nitrophenoxy)acetohydrazide

752383

N'-benzoyl-2,3-dichlorobenzohydrazide

N'-benzoyl-2,4-dichlorobenzohydrazide

N'-benzoyl-3-chlorobenzohydrazide

N'-benzoyl-3-methylbenzohydrazide

N'-benzoyl-4-chlorobenzohydrazide

N'-benzoyl-4-methoxybenzohydrazide

N'-benzoyl-4-methylbenzohydrazide

N'-benzoylbenzohydrazide

N'-benzoylpyridine-3-carbohydrazide

N'-[[8-methyl-2,4-dioxo-2H-pyrido[1,2-a]pyrimidin-3(4H)-ylidene]methyl]-benzo-hydrazide

13% inhibition at 0.01 mM

N-(1,3-benzothiazol-2-yl)phosphoric triamide

687255

N-(2,3-dimethylphenyl)-5-phenyl-1,3,4-thiadiazol-2-amine

N-(2,4-dichlorophenyl)-N'-(3,4-dimethylphenyl)thiourea

N-(2,4-dichlorophenyl)-N'-(3,5-dimethylphenyl)thiourea

N-(2,4-dichlorophenyl)-N'-(4-ethylphenyl)thiourea

N-(2,4-difluorophenyl)-N'-(2-iodophenyl)thiourea

N-(2,4-difluorophenyl)-N'-(3,4-dimethylphenyl)thiourea

N-(2,4-difluorophenyl)-N'-(3,5-dimethylphenyl)thiourea

N-(2,4-difluorophenyl)-N'-(4-iodophenyl)thiourea

N-(2,4-dimethylphenyl)-5-(3-nitrophenyl)-1,3,4-thiadiazol-2-amine

N-(2,5-dimethylphenyl)-N'-(3-methoxyphenyl)thiourea

N-(2,5-dimethylphenyl)-N'-[3-(trifluoromethyl)phenyl]thiourea

N-(2-bromophenyl)-N'-(4-ethylphenyl)thiourea

N-(2-bromophenyl)-N'-(5-chloro-2,4-dimethoxyphenyl)thiourea

N-(2-bromophenyl)-N'-[2-hydroperoxy-5-(trifluoromethyl)phenyl]thiourea

N-(2-bromophenyl)-N'-[3-(methylsulfanyl)phenyl]thiourea

N-(2-chloro-4,6-dimethoxyphenyl)-N'-(3-chlorophenyl)thiourea

N-(2-chloro-4,6-dimethoxyphenyl)-N'-[3-(trifluoromethyl)phenyl]thiourea

N-(2-iodophenyl)-N'-(3-methoxyphenyl)thiourea

N-(2-methoxy-4-nitrophenyl)phosphoric triamide

N-(2-nitrophenyl)phosphoric triamide

687255, 698419

N-(2-phenylethyl)phosphoric triamide

N-(2-phenylethyl)phosphorothioic triamide

N-(3,4-dimethylphenyl)-N'-[2-(trifluoromethyl)phenyl]thiourea

N-(3,4-dimethylphenyl)-N'-[2-hydroperoxy-5-(trifluoromethyl)phenyl]thiourea

N-(3,4-dimethylphenyl)-N'-[3-(trifluoromethyl)phenyl]thiourea

N-(3,5-dimethylphenyl)-N'-(3-methoxyphenyl)thiourea

N-(3-chlorophenyl)-N'-(3-nitrophenyl)thiourea

N-(3-chlorophenyl)-N'-(4-methoxyphenyl)thiourea

N-(3-chlorophenyl)-N'-[2-methoxy-5-(trifluoromethyl)phenyl]thiourea

N-(3-methoxyphenyl)-N'-(3-nitrophenyl)thiourea

N-(3-methoxyphenyl)phosphoric triamide

N-(3-methylpyridin-2-yl)phosphorothioic triamide

N-(3-morpholin-2-ylpropyl)phosphoric triamide

N-(3-nitropyridin-2-yl)phosphoric triamide

N-(4-bromophenyl)-N'-(2,4-difluorophenyl)thiourea

N-(4-bromophenyl)-N'-(3-chlorophenyl)thiourea

N-(4-bromophenyl)-N'-(3-fluorophenyl)thiourea

N-(4-bromophenyl)-N'-(3-methoxyphenyl)thiourea

N-(4-chlorophenyl)-N'-[2-(trifluoromethyl)phenyl]thiourea

N-(4-cyclohexylphenyl)phosphoric triamide

N-(4-ethylphenyl)-N'-(3-methoxyphenyl)thiourea

N-(4-ethylphenyl)-N'-[3-(trifluoromethyl)phenyl]thiourea

N-(4-fluoro-3-nitrophenyl)-N'-(3-methoxyphenyl)thiourea

N-(4-iodophenyl)-N'-(3-methoxyphenyl)thiourea

N-(4-iodophenyl)-N'-[3-(trifluoromethyl)phenyl]thiourea

N-(4-methoxy-1,3-benzothiazol-2-yl)phosphoric triamide

N-(4-methyl-1,3-benzothiazol-2-yl)phosphoric triamide

N-(4-methyl-2-nitrophenyl)phosphoric triamide

687255, 698419

N-(4-nitrophenyl)phosphoric triamide

N-(4-phenoxyphenyl)phosphoric triamide

N-(5-chloro-2,4-dimethoxyphenyl)-N'-(3-methoxyphenyl)thiourea

N-(5-chloro-2,4-dimethoxyphenyl)-N'-[2-(trifluoromethyl)phenyl]thiourea

N-(5-chloro-2-methylphenyl)-N'-(3,4-dichlorophenyl)thiourea

N-(6-ethoxy-1,3-benzothiazol-2-yl)phosphoric triamide

N-(6-fluoro-1,3-benzothiazol-2-yl)phosphoric triamide

N-(benzylcarbamothioyl)-2-[4-(2-methylpropyl)phenyl]propanamide

N-(N'-benzyloxycarbonylglycyl)aminomethyl(P-methyl)phosphinothioic acid

N-1,3-benzothiazol-2-ylphosphoric triamide

N-1,3-benzothiazol-2-ylphosphorothioic triamide

N-adamant-1-ylphosphoric triamide

N-benzyloxycarbonylaminomethyl(P-methyl)phosphinic acid

N-dimethyl-L-cysteine

N-dimethyl-S-methyl-L-cysteine

N-ethylmaleimide

irreversible inactivation

699229

N-glycyl-aminomethyl(P-methyl)phosphinic acid

N-hydroxyacetamide

N-n-butylphosphoric triamide

N-n-butylthiophosphoric triamide

687255, 698419

N-naphthalen-1-ylphosphoric triamide

N-phenylphosphoric triamide

N-[(2,3-dichlorophenyl)carbamothioyl]-2-[4-(2-methylpropyl)phenyl]propanamide

N-[(2,4-dinitrophenyl)carbamothioyl]-2-[4-(2-methylpropyl)phenyl]propanamide

N-[(3,5-dinitrophenyl)carbamothioyl]-2-[4-(2-methylpropyl)phenyl]propanamide

N-[(4-bromophenyl)carbamothioyl]-2-[4-(2-methylpropyl)phenyl]propanamide

N-[(4-chlorophenyl)carbamothioyl]-2-[4-(2-methylpropyl)phenyl]propanamide

N-[(4-methoxyphenyl)carbamothioyl]-2-[4-(2-methylpropyl)phenyl]propanamide

N-[2-(4-fluorophenyl)ethyl]phosphoric triamide

N-[2-(difluoromethoxy)phenyl]phosphoric triamide

N-[2-(trifluoromethoxy)phenyl]phosphoric triamide

N-[3-(methylsulfanyl)phenyl]-N'-[2-(trifluoromethyl)phenyl]thiourea

N-[4-(benzyloxy)phenyl]phosphoric triamide

N-[[hydroxy(methyl)phosphoryl]methyl]-L-phenylalaninamide

N-[[hydroxy(methyl)phosphoryl]methyl]-L-serinamide

N-[[hydroxy(methyl)phosphoryl]methyl]-L-valinamide

N2-[(benzyloxy)carbonyl]-N-[[hydroxy(methyl)phosphoryl]methyl]glycinamide

NaF

NEM

Ni2+

O-benzyl-N-[[hydroxy(methyl)phosphoryl]methyl]-L-serinamide

O-[4-(benzyloxy)phenyl] diamidothiophosphate

oxovanadium complex [VOL(AHA)]

reaction of N'-(3-bromo-2-hydroxybenzylidene)-4-methoxybenzohydrazide and acetohydroxamic acid with VO(acac)2 in methanol gives the complex [VOL(AHA)]. The benzohydrazone ligand, in its dianionic form, coordinates to V atom through the phenolate oxygen, imino nitrogen and enolate oxygen. The acetohydroxamic acid coordinates to V atom through the carbonyl oxygen and deprotonated hydroxyl oxygen. The V atom is in octahedral coordination. The percent inhibition at concentration of 0.1 mM/l on Helicobacter pylori urease is 78% for the oxovanadium complex

752942

p-benzoquinone

688020

p-chloromercuribenzene sulfonate

Bos taurus

0.1 mM, complete inhibition

209162

p-hydroxymercuribenzoate

Pb2+

Corynebacterium ammoniagenes

inhibitory effect of heavy metals over immobilized enzyme decreases in the order Cu2+, Cd2+, Zn2+, Ni2+, Pb2+

670117

PCMB

0.1 mM, complete loss of activity

209155

peptides

TFLPQPRCSALLRYLSEDGVIVPS and YDFYWW, no inhibition of the enzyme from Bacillus pasteurii and Canavalia ensiformis

209168

phenylboronic acid

Mycobacterium tuberculosis

phenylphosphoramidate

209182

phenylphosphorodiamidate

Staphylococcus saprophyticus

Phenylurea

weak non-competitive

Phosphoramidate

psi-tectorigenin

0.4 mg/ml, 36% inhibition

quercetin

quercetin-4'-O-beta-D-glucopyranoside

quercitrin

S-ethyl-L-cysteine

Selenourea

50 mM, 80% inhibition

Semicarbazide

silver nanoparticles

Sr2+

sulfite

competitive inhibitor. The structure of the urease-sulfite complex, determined at 1.65 A resolution, shows the inhibitor bound to the dinuclear Ni(II) center of urease in a tridentate mode involving bonds between the two Ni(II) ions in the active site and all three oxygen atoms of the inhibitor

754342

tetrachloro-o-benzoquinone

no recovery of urease activity bound in the urease-inhibitor complex after dilution or addition of dithiothreitol

688019

tetrachloro-p-benzoquinone

688019

tetracosyl (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoate

712598

Thiourea

15 entries

triphenylbismuth difluoride

first-order rate constant for inactivation 0.00158 per s

tris(2,4,6-trimethylphenyl)bismuth

first-order rate constant for inactivation 0.0027 per s

tris(4-fluorophenyl)bismuth dichloride

first-order rate constant for inactivation 0.00158 per s

tris(4-fluorophenyl)bismuth difluoride

first-order rate constant for inactivation 0.00259 per s

tris(4-methylphenyl)bismuth dichloride

first-order rate constant for inactivation 0.00259 per s

Staphylococcus saprophyticus

Urea

competitive

209178

Zn2+

5 entries

[(acetylamino)methyl]methylphosphinic acid

[(acetylamino)methyl]phosphonic acid

718558

[(benzylamino)methyl]methylphosphinic acid

[(dimethylamino)methyl]methylphosphinic acid

[(dimethylamino)methyl]phosphonic acid

[(methylamino)methyl]phosphonic acid

[5-[(4-methylphenyl)amino]-1,3,4-oxadiazol-2-yl](phenyl)methanol

[5-[(4-nitrophenyl)amino]-1,3,4-oxadiazol-2-yl](phenyl)methanol

[[benzyl(methyl)amino]methyl]phosphonic acid