3.5.1.36: N-methyl-2-oxoglutaramate hydrolase

This is an abbreviated version!
For detailed information about N-methyl-2-oxoglutaramate hydrolase, go to the full flat file.

Reaction

N-Methyl-2-oxoglutaramate
+
H2O
=
2-oxoglutarate
+
methylamine

Synonyms

5-hydroxy-N-methylpyroglutamate synthase, 5-hydroxy-N-methylpyroglutamate synthetase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.36 N-methyl-2-oxoglutaramate hydrolase

Purification

Purification on EC 3.5.1.36 - N-methyl-2-oxoglutaramate hydrolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
extraction, addition of streptomycin sulfate, adjust the pH value, centrifugation, ammonium sulfate precipitation, dialysis, purification with DEAE, Sephadex and hydroxyapatite column with dialysis and ultrafiltration steps
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