3.5.1.29: 2-(acetamidomethylene)succinate hydrolase

This is an abbreviated version!
For detailed information about 2-(acetamidomethylene)succinate hydrolase, go to the full flat file.

Word Map on EC 3.5.1.29

Reaction

2-(Acetamidomethylene)succinate
+ 2 H2O =
acetate
+
succinate semialdehyde
+
NH3
+
CO2

Synonyms

alpha-(N-acetylaminomethylene)succinic acid hydrolase, compound A hydrolase, alpha-(N-acetylaminomethylene)succinic acid amidohydrolase, AAMSAH, AAMS amidohydrolase, (E)-2-(acetamidomethylene)succinate hydrolase, E-2AMS hydrolase, mlr6787

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.29 2-(acetamidomethylene)succinate hydrolase

Crystallization

Crystallization on EC 3.5.1.29 - 2-(acetamidomethylene)succinate hydrolase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 9% (w/v) PEG 8000, 200 mM MgCl2, and 100 mM Tris at pH 7.8
-
sitting drop vapor diffusion method, using 100 mM Tris-HCl buffer (final pH 8.5), 20% (w/v) PEG 8000, 275 mM MgCl2