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3.5.1.28: N-acetylmuramoyl-L-alanine amidase

This is an abbreviated version!
For detailed information about N-acetylmuramoyl-L-alanine amidase, go to the full flat file.

Word Map on EC 3.5.1.28

Reaction

GlcNAc-MurNAc-L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala
+
H2O
=
N-acetylglucosaminyl-N-acetylmuramic acid
 +
L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala

Synonyms

1,6-anhydro-N-acetylmuramic acid-L-alanine amidase, 1,6-anhydro-N-acetylmuramyl-L-alanine amidase, acetylmuramoyl-alanine amidase, acetylmuramyl-alanine amidase, acetylmuramyl-L-alanine amidase, AMI1, AmiA, AmiB, AmiC, AmiC2, AmiD, amidase 3, amidase-hydrolase, AmiE, AmpD, anhydroMurNAc-L-Ala amidase, AtlE, Autolysin, autolysin E, bacteriophage phiGVE2 amidase, cell separation amidase, cell wall amidase, Cell wall hydrolase, CwhA, CwlB protein, cwlC, CwlJ1, EC 3.4.17.7, EC 3.4.19.10, endolysin, GC-AmiC, GRCS_0011, LysBPS13, lysostaphin, LysSA97, LytA, LytA-like N-acetylmuramoyl-L-alanine amidase, LytAB6, LytAHER, Lytic amidase, Lytmu1/6, LytN, More, MSMEG_6281, Mtb peptidoglycan amidase, Mucopeptide aminohydrolase, murein hydrolase, MurnAc-lAA, Nacetylmuramoyl-L-alanine amidase activity, N-acetyl muramyl-L-alanine amidase, N-acetyl-muramoyl-L-alanine amidase, N-acetylmuramic acid L-alanine amidase, N-acetylmuramoyl-L-alanine amidase, N-acetylmuramoyl-l-alanine amidase B, N-acetylmuramoyl-L-alanine amidase type I, N-acetylmuramoyl-L-alanine amidase type II, N-acetylmuramyl-L-alanine amidase, N-acetylmuramylalanine amidase, N-acylmuramyl-L-alanine amidase, NAM-amidase, NAMLA amidase, NAMLAA, Npun_F1846, ORFL3, peptidoglycan amidase, peptidoglycan aminohydrolase, Peptidoglycan hydrolase, peptidoglycan recognition protein 2, peptidoglycan recognition protein SC1a, peptidoglycan recognition protein-L, PG amidase, PGLYRP2, PGRP, PGRP-L, PGRP-S4, PGRP-SB1, phage endolysin, phi26F_gp22, phiCP26F endolysin, phiGVE2 endolysin, PlyCP26F, PlyCP39O, PlyCpAmi, PlyGRCS, PlyPl23, RC0497, RS15875, Rv3717, SA97_036, short peptidoglycan recognition protein 4, Skl, Sle1, SleC, spore cortex lytic enzyme, T3 lysozyme, T7 lysozyme

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.28 N-acetylmuramoyl-L-alanine amidase

Temperature Stability

Temperature Stability on EC 3.5.1.28 - N-acetylmuramoyl-L-alanine amidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
10 min at 25°C result in 25% loss of inactivation
35
-
Tm, H34A mutant AmpD + Zn2+
39
-
Tm, D164A mutant AmpD + Zn2+
4 - 100
-
the enzyme demonstrates remarkable thermostability in the presence of 30% (v/v) glycerol, and it retains its lytic activity after incubation between 4 and 100°C for 30 min
4 - 22
PlyCP26F and PlyGVE2CpCWB are completely stable for at least 30 min
4 - 42
recombinant chimeric endolysin PlyGVE2CpCWB is completely stable for at least 30 min
42
PlyCP26F loses roughly 40% of its activity after 30 min at 42°C, while PlyGVE2CpCWB is fully active
46
-
Tm, D164A mutant AmpD
5
-
half-life of staphylolytic activity: approximately 2 months (pH 4)
51
-
Tm, H34A mutant AmpD
52
-
Tm, H154A mutant AmpD + Zn2+
56
-
Tm, H154A mutant AmpD, K162H mutant AmpD + Zn2+
57
-
Tm, H154N mutant AmpD + Zn2+
59
-
Tm, K162Q mutant AmpD + Zn2+
61
-
Tm, Y63F mutant AmpD
62
-
Tm, E116A mutant AmpD + Zn2+, K162H mutant AmpD
65
recombinant chimeric endolysin PlyGVE2CpCWB, 10% activity remaining after 30 min, inactivation of PlyCP26F
66
-
Tm, E116A mutant AmpD
70
A0A1J9W3F9
purified recombinant His-tagged enzyme, 50 min, 50% activity remaining
95
A0A1J9W3F9
purified recombinant His-tagged enzyme, loss of about 95% activity
additional information
PlyGVE2CpCWB is more tolerant to heat treatment than PlyCP26F