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3.5.1.28: N-acetylmuramoyl-L-alanine amidase

This is an abbreviated version!
For detailed information about N-acetylmuramoyl-L-alanine amidase, go to the full flat file.

Word Map on EC 3.5.1.28

Reaction

GlcNAc-MurNAc-L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala
+
H2O
=
N-acetylglucosaminyl-N-acetylmuramic acid
 +
L-Ala-D-isoglutaminyl-meso-diaminopimelyl-D-Ala-D-Ala

Synonyms

1,6-anhydro-N-acetylmuramic acid-L-alanine amidase, 1,6-anhydro-N-acetylmuramyl-L-alanine amidase, acetylmuramoyl-alanine amidase, acetylmuramyl-alanine amidase, acetylmuramyl-L-alanine amidase, AMI1, AmiA, AmiB, AmiC, AmiC2, AmiD, amidase 3, amidase-hydrolase, AmiE, AmpD, anhydroMurNAc-L-Ala amidase, AtlE, Autolysin, autolysin E, bacteriophage phiGVE2 amidase, cell separation amidase, cell wall amidase, Cell wall hydrolase, CwhA, CwlB protein, cwlC, CwlJ1, EC 3.4.17.7, EC 3.4.19.10, endolysin, GC-AmiC, GRCS_0011, LysBPS13, lysostaphin, LysSA97, LytA, LytA-like N-acetylmuramoyl-L-alanine amidase, LytAB6, LytAHER, Lytic amidase, Lytmu1/6, LytN, More, MSMEG_6281, Mtb peptidoglycan amidase, Mucopeptide aminohydrolase, murein hydrolase, MurnAc-lAA, Nacetylmuramoyl-L-alanine amidase activity, N-acetyl muramyl-L-alanine amidase, N-acetyl-muramoyl-L-alanine amidase, N-acetylmuramic acid L-alanine amidase, N-acetylmuramoyl-L-alanine amidase, N-acetylmuramoyl-l-alanine amidase B, N-acetylmuramoyl-L-alanine amidase type I, N-acetylmuramoyl-L-alanine amidase type II, N-acetylmuramyl-L-alanine amidase, N-acetylmuramylalanine amidase, N-acylmuramyl-L-alanine amidase, NAM-amidase, NAMLA amidase, NAMLAA, Npun_F1846, ORFL3, peptidoglycan amidase, peptidoglycan aminohydrolase, Peptidoglycan hydrolase, peptidoglycan recognition protein 2, peptidoglycan recognition protein SC1a, peptidoglycan recognition protein-L, PG amidase, PGLYRP2, PGRP, PGRP-L, PGRP-S4, PGRP-SB1, phage endolysin, phi26F_gp22, phiCP26F endolysin, phiGVE2 endolysin, PlyCP26F, PlyCP39O, PlyCpAmi, PlyGRCS, PlyPl23, RC0497, RS15875, Rv3717, SA97_036, short peptidoglycan recognition protein 4, Skl, Sle1, SleC, spore cortex lytic enzyme, T3 lysozyme, T7 lysozyme

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.28 N-acetylmuramoyl-L-alanine amidase

Inhibitors

Inhibitors on EC 3.5.1.28 - N-acetylmuramoyl-L-alanine amidase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
benzoyl-Arg-p-nitroanilide
-
inhibition: moderately at 1 mM, completely at 10 mM
beta-mercaptoethanol
-
-
CDTA
-
about 90% inhibition; enzyme results in about 90% loss of activity, when dialyzed overnight against the metal chelator. Some restoration of activity is observed with all of the metal salts (10 mM ZnCl2, MgCl2, CaCl2, CoCl2, or NiCl). The addition of ZnCl2, MgCl2, and CaCl2 is the most effective, restoring 70-80% of the activity, with NiCl2 restoring about 50% and CoCl2 the least effective at only about 25%
choline
CTAB
-
almost complete inhibition at 0.1 (v/v)
DD-diaminopimelic acid
-
at 10 mM
deoxycholate
at 1%; at 1%
dipicolinic acid
-
0.005 mM, complete inhibition after 30 min, activity can be restored by 0.1 mM ZnSO4
dithiothreitol
glutathione
-
-
iodoacetamide
-
-
iodoacetate
-
-
KCl
-
at a concentration of 200 mM
L-alanyl-gamma-D-glutamyl-(L)-meso-diaminopimelic acid
-
at 5 mM
LiCl
-
at a concentration of 200 mM
lipoteichoic acid
-
meso-diaminopimelic acid
-
at 10 mM
MgCl2
-
at a conecentration of 1 mM
Muramic acid
-
-
MurNAc-L-Ala-D-Gln
-
competitive inhibitor
-
N-acetylglucosamine
-
-
N-acetylmuramic acid
-
-
p-chloromercuribenzene sulfonate
p-hydroxymercuribenzoic acid
Dubowvirus dv11
-
-
phosphatidylglycerol
SDS
-
almost complete inhibition at 0.1 (v/v)
Tris-HCl
lytic activity in 30 mM Tris-HCl is only 30% of that in 10 mM solution, almost inactive in 70 mM Tris-HCl
additional information
-