3.5.1.26: N4-(beta-N-acetylglucosaminyl)-L-asparaginase

This is an abbreviated version!
For detailed information about N4-(beta-N-acetylglucosaminyl)-L-asparaginase, go to the full flat file.

Reaction

Synonyms

1-aspartamido-beta-N-acetylglucosamine amidohydrolase, 4-L-aspartylglucosylamine amido hydrolase, AGA, amidase-1, amidase-2, amidase-3, aspartylglucosaminidase, aspartylglucosylaminase, aspartylglucosylamine deaspartylase, aspartylglycosylamine amidohydrolase, AtAGA, beta-aspartylglucosylamine amidohydrolase, EC 3.5.1.37, GA, glucosylamidase, glycoasparaginase, glycosylasparaginase, LhAGA, More, N-aspartyl-beta-glucosaminidase, N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.26 N4-(beta-N-acetylglucosaminyl)-L-asparaginase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
8	Activating Compound
421	AA Sequence
10	Application
1	CAS Registry Number
12	Cloned(Commentary)
7	Crystallization (Commentary)
273	Disease/ Diagnostics
21	General Information
8	General Stability
46	Inhibitors
3	kcat/KM [mM/s]
14	Ki Value [mM]
34	KM Value [mM]
28	Localization
38	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
48	Organism
2	Pathway
38	PDB ID
20	pH Optimum
5	pH Range
3	pH Stability
27	Posttranslational Modification
53	Protein Variants
27	Purification (Commentary)
3	Reaction
1	Reaction Type
48	Reference
47	Source Tissue
21	Specific Activity [micromol/min/mg]
4	Storage Stability
73	Substrates and Products (Substrate)
29	Subunits
58	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
10	Temperature Stability [°C]
7	Turnover Number [1/s]

Cloned

print visible entries

print all entries

Cloned on EC 3.5.1.26 - N4-(beta-N-acetylglucosaminyl)-L-asparaginase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Cloned (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

DNA sequence determination and analysis, overexpression of mutant L15R in BHK and COS-1 cells, low expression level in endoplasmic reticulum, the recombinant active enzyme does not reach the lysosomes

Homo sapiens

P20933

668879

expression in COS cells

Homo sapiens

-

209048, 209051, 209052

expression in COS-1 cells and in BHK-21 cells

Homo sapiens

P20933

654537

expression in Escherichia coli

Elizabethkingia meningoseptica

-

209049

expression in NIH-3T3 cells

Homo sapiens

-

209047

gene AGA, DNA and amino acid sequence determination and analysis, genomic organization of Aurelia gene AGA and exon-intron structure, molecular phylogenetic analysis based on the nucleotide sequences of N-terminal nucleophile (Ntn)_asparaginase_2_like superfamily genes, quantitative real-time PCR enzyme expresssion analysis

Aurelia aurita

-

753910

gene AGA, DNA and amino acid sequence determination and analysis, sequence comparisons with human enzyme and Asobara tabida enzyme

Leptopilina heterotoma

A0A141NXG8

755152

gene AGA, DNA and amino acid sequence determination and analysis, sequence comparisons with human enzyme and Leptopilina heterotoma enzyme

Asobara tabida

D0V0N4

755152

gene AGA, genotyping, recombinant expression of codon-optimized genes encoding enzyme variants S149 and T149 in HEK-293T and HeLa cells, the Thr149 variant exhibits a slightly higher expression level than the Ser149 variant. Recombinant expression of AGA variants in transgenic patient fibroblasts, both wild-type and AGUFin-major mutant type. The transfection with the AGA variants improves the lysosomal morphology in AGU fibroblasts, low transfection efficiency

Homo sapiens

P20933

753910

recombinant expression of wild-type and mutant enzymes

Elizabethkingia miricola

Q47898

735290