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3.5.1.24: choloylglycine hydrolase

This is an abbreviated version!
For detailed information about choloylglycine hydrolase, go to the full flat file.

Word Map on EC 3.5.1.24

Reaction

glycocholate
+
H2O
=
cholate
 +
glycine

Synonyms

BAH1, BFS26_06805, bile acid hydrolase, bile salt hydrolase, Bile-Salt-Hydrolase, BSH, BSH A, BSH B, BSH C, BSH1, BSH12, BSH2, BSH3, BSH4, BSH47, BSH56, BSHA, BSHB, BSHC, BT2086, C3745_01535, CBAH, CBH, CBSHalpha, CBSHbeta, CGH, cholylglycine hydrolase, Conjugated bile acid hydrolase, conjugated bile salt hydrolase, conjugated bile salt hydrolase alpha peptide, conjugated bile salt hydrolase beta, EFBG_01849, EfBSH, glycocholase, LaciP, LgBSH, linear amide C-N hydrolase, LJ1412, LJ_0056, LJ_1147, LsalN1, lsBSH, More, probiotic bile salt hydrolase, salt hydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.24 choloylglycine hydrolase

Purification

Purification on EC 3.5.1.24 - choloylglycine hydrolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
bile acid hydrolase A, B, C and D
-
native enzyme 21.6fold from strain KL412 to homogeneity by hydrophobic interaction and anion exchange chromatography, and gel filtration
-
native enzyme 33.5fold from strain ATCC 11863 to homogeneity by hydrophobic interaction and anion exchange chromatography, and gel filtration
-
native enzyme by gel filtration, hydrophobic interaction chromatography, and ion exchange chromatography
native enzyme from strains ATCC 15708, KL507, and KL515 to homogeneity by hydrophobic interaction and anion exchange chromatography, and gel filtration, 30.3fold from strain KL515
-
nickel-based affinity chromatography
-
purified to homogeneity using Q-Sepharose chromatography
-
Q-Sepharose column chromatography and Sephacryl S-300 gel filtration
recombinant BSH from strain PF01 purifed from recombinant Escherichia coli strrain BL21(DE3)
-
recombinant enzyme expressed in Escherichia coli
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography, and polymyxin B resin chromatography, the latter to eliminate lipopolysaccharide contamination
recombinant expression of His-tagged enzyme from Escherichia coli strain BL21(DE3) clone (JL 885) by nickel affinity chromatography
recombinant His-tagged BSH from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange and hydrophobic interaction chromatography, and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography, partially
recombinant His-tagged isozyme BSH1 from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography, ultrafiltration, and gel filtration
-
recombinant His-tagged isozyme BSH2 from Escherichia coli strain BL21(DE3) by immobilized metal affinity chromatography, ultrafiltration, and gel filtration
-
recombinant His-tagged isozyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration to apparent homogeneity
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nicke affinity chromatography to homogeneity
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant soluble His-tagged enzyme 39.34fold from Escherichia coli strain BL21(DE3) pLysS by ammonium sulfate fractionation, nickel affinity chromatography, and gel filtration, to homogeneity
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
-
recombinant wild-type and mutant enzymes from Escherichia coli strain JL885