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3.5.1.24: choloylglycine hydrolase

This is an abbreviated version!
For detailed information about choloylglycine hydrolase, go to the full flat file.

Word Map on EC 3.5.1.24

Reaction

glycocholate
+
H2O
=
cholate
 +
glycine

Synonyms

BAH1, BFS26_06805, bile acid hydrolase, bile salt hydrolase, Bile-Salt-Hydrolase, BSH, BSH A, BSH B, BSH C, BSH1, BSH12, BSH2, BSH3, BSH4, BSH47, BSH56, BSHA, BSHB, BSHC, BT2086, C3745_01535, CBAH, CBH, CBSHalpha, CBSHbeta, CGH, cholylglycine hydrolase, Conjugated bile acid hydrolase, conjugated bile salt hydrolase, conjugated bile salt hydrolase alpha peptide, conjugated bile salt hydrolase beta, EFBG_01849, EfBSH, glycocholase, LaciP, LgBSH, linear amide C-N hydrolase, LJ1412, LJ_0056, LJ_1147, LsalN1, lsBSH, More, probiotic bile salt hydrolase, salt hydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.24 choloylglycine hydrolase

Cloned

Cloned on EC 3.5.1.24 - choloylglycine hydrolase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence comparison, phylogenetic analysis and tree
DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) clone (JL 885)
DNA and amino acid sequence determination and analysis, two different genetic elements corresponding to each of the enzymatic activity are successfully amplified from the genomic DNA of the isolate, phylogenetic analysis
KJ571489
DNA and amino acid sequence determination, analysis, and comparison of BSH from strain PF01, expression in Escherichia coli strain BL21(DE3)
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expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli XL1 Blue cells
expression in Escherichia coli
gene bsh, DNA and amino acid sequence determination and analysis, real-time PCR expression analysis
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gene bsh, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21
gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL1-Blue
gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Eschericchia coli strain DH5alpha
gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL1-Blue
gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene bsh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain DH5alpha intracellularly in absence of bile salts
gene bsh, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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gene bsh, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JL885
gene bsh, sequence comparisons and phylogenetic analysis, recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strains BLR(DE3) and XL-1 Blue
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gene bsh, sequence comparisons, recombinant expression of wild-type and mutant C-terminally His-tagged enzyme in Escherichia coli strain BL21
gene bsh1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL-1 Blue
gene bsh1, recombinant expression in Lactococcus lactis strain NZ9000 from plasmid pNZ8148, subcloning in Escherichia coli, the resulting Lactococcus lactis strain NZ-bsh1 shows a 2.8fold increased biomass compared to wild-type
gene bsh1, recombinant overexpression in enzyme-deficient Lactobacillus casei LC2W, the transformed strain is termed Lactobacillus casei pWQH01, quantitative real-time PCR gene expression analysis
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gene bsh12 or LJ1412, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of His-tagged enzyme in Escherichia coli strain SE1, subcloning in Escherichia coli strain CYS21
gene bsh2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL-1 Blue
gene bsh2, recombinant expression in Lactococcus lactis strain NZ9000 from plasmid pNZ8148, subcloning in Escherichia coli, the resulting Lactococcus lactis strain NZ-bsh2 shows a 2.4fold increased biomass compared to wild-type
gene bsh3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL-1 Blue
gene bsh4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BLR(DE3), subcloning in Escherichia coli strain XL-1 Blue
gene bsh47 or LJ1147, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of His-tagged enzyme in Escherichia coli strain SE1, subcloning in Escherichia coli strain CYS21
gene bsh56 or LJ1156, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant overexpression of His-tagged enzyme in Escherichia coli strain SE1, subcloning in Escherichia coli strain CYS21
gene cgh, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain BL21(DE3)
gene PF01_00515, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged isozyme in Escherichia coli strain BLR(DE3)
gene PF01_00682, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged isozyme in Escherichia coli strain BLR(DE3)
gene PF01_01578, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged isozyme in Escherichia coli strain BLR(DE3)
genes bsh are cloned and expressed in Escherichia coli
-
into pCR-Blunt and subsequently into pNZ8048 for heterologous overexpression of BSH in Lactococcus lactis cells
isolates of lactic acid bacteria (LAB) from flowers are genotyped and screened for the bile salt hydrolase activity on agar supplemented with 0.5% w/v taurodeoxycholic acid. The isolates are divided into two groups based on their phenotypic characteristics and 16S rRNA gene sequence analysis of the representative isolates. DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
isolates of lactic acid bacteria (LAB) from flowers are genotyped and screened for the bile salt hydrolase activity on agar supplemented with 0.5% w/v taurodeoxycholic acid. The isolates are divided into two groups based on their phenotypic characteristics and 16S rRNA gene sequence analysis of the representative isolates. DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis. Group I consisted of 10 isolates, which were FM1-1, FM1-2, FM2-3, FM4-1, FM4-2, FM11-1, FM11-2, FM11-3, FM12-1, and FM12-2. Group II consisted of six isolates, which are FM3-1, FM13-1, FM14-1, FM14-2, FM16-1, and FM16-2. Eleven isolates, including FM1-1, FM1-2, FM2-3, FM3-1, FM4-2, FM11-2, FM12-1, FM12-2, FM14-1, FM14-2, and FM16-2, exhibit bile salt hydrolase activity. All LAB isolates show the cholesterol assimilated ability ranged from 9.57% to 51.69%. The isolate FM11-2 efficiently assimilated the cholesterol with 51.69%
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isozyme BSH1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) , subcloning in Escherichia coli strain JM109
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isozyme BSH2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109
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Lactobacilli bsh DNA and amino acid sequence comparisons, phylogenetic analysis, overview
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phylogenetic tree
recombinant expression of C-terminally His-tagged BSH in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS and usage of the twin-arginine translocation (Tat) pathway as an alternative for this secretory production, enzyme secretion by the twin-arginine signal peptide of DMSO reductase subunit DmsA from Escherichia coli, method, overview. Most of the enzyme is expresed in intracellular inclusion bodies, but some is secreted to the cell culture medium
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vector-free engineering by chromosomal integration of an exogenous cbh gene, encoding the conjugated BSH, is cloned and expressed in Lactobacillus casei strain LK1 with the aid of pSMA23-derived vectors
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