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3.5.1.2: glutaminase

This is an abbreviated version!
For detailed information about glutaminase, go to the full flat file.

Word Map on EC 3.5.1.2

Reaction

L-glutamine
+
H2O
=
L-glutamate
 +
NH3

Synonyms

AnsB, AoGls, GA, GAB, GAC, GahB, GLA, GLNase, GLS, GLS1, GLS2, GlsA, glutaminase, glutaminase 1, glutaminase 2, glutaminase A, glutaminase B, glutaminase C, glutaminase I, glutaminase K, glutaminase L, glutaminase-1, glutaminase-2, glutaminase-B, glutamine amidohydrolase, glutamine aminohydrolase, glutamine deamidating enzyme, K-glutaminase, KAG, KGA, kidney-type glutaminase, kidney-type-glutaminase, L-glutaminase, L-glutamine amidohydrolase, LAG, LGA, liver-type glitaminase, liver-type glutaminase, Mglu, Micrococcus glutaminase Mglu, Micrococcus luteus K-3-type glutaminase, mitochondrial glutaminase, N-PAG, neuroblastoma glutaminase, neuroblastoma PAG, Nit 2, nitrilase 2, omega-amidase, PAG, PDX2, phosphate activated glutaminase, phosphate-activated glutaminase, phosphate-activated L-glutamine amidohydrolase, salt-tolerant glutaminase, YaaE

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.2 glutaminase

Purification

Purification on EC 3.5.1.2 - glutaminase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
acetone precipitation and carboxymethyl-methacrylate column chromatography
-
by centrifugation, lysis, nickel affinity chromatography
-
by two-step ammonium sulfate precipitation, hydrophobic interaction chromatography on phenyl-Sepharose, anion exchange chromatography on DEAE-cellulose, hydrophobic interaction chromatography on phenyl-Superose and anion exchange chromatography using Mono Q, 762fold
-
DEAE Sepharose column chromatography, hydroxyapatite column chromatography, and Sephacryl S200 gel filtration
-
DEAE-cellulose column chromatography
-
DEAE-Toyopearl column
-
glutaminase B
-
ion exchange column chromatography and gel filtration
-
native enzyme 250fold from strain NYW-81 to homogeneity by two steps of anion exchange chromatography, hydrophobic interaction and hydroxylapatite chromatography, and gel filtration
-
native enzyme from strain K-3 to homogeneity, recombinant enzyme from Escherichia coli strain JM109 by anion exchange chromatography to homogeneity
-
native enzyme, 49fold from cell-free extract by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, to homogeneity
-
Ni+-charged affinity column chromatography
-
Ni-NTA column chromatpgraphy and gel filtration
Ni2+-NTA column chromatography
partially from rat pancreas by preparation of islets of Langerhans
-
partially purified by heating and Sephadex G-100 gel filtration, 25% yield
-
partially purified, 21fold, 0.2% yield, protamine sulfate treatment, anion exchange chromatography, and gel filtration
-
purification of glutaminase I, partial purification of glutaminase II
-
recombinant C-terminally His6-tagged wild-type and mutant A47Q enzymes from Escherichia coli strain BL21(DE3) cytosol by nickel affinity chromatography
-
recombinant enzyme
-
recombinant enzyme 5.9fold from Escherichia coli by anion exchange, glutamine affinity, and hydrophobic interaction chromatography to homogeneity
recombinant enzyme with N-terminal deletion and recombinant enzyme with N-terminal and C-terminal deletions, by nickel-affinity chromatography, 63fold and 113fold respectively
-
recombinant enzyme, by metal affinity chromatography
-
recombinant His-tagged TrpG, and mutant TrpG variants T129F and T129A from Escherichia coli strain BL21(DE3)Rosetta by heat treatment for 20 min at 60°C, nickel affinity chromatography, and anion exchange chromatography
-
recombinant His10-tagged GlsA 2.7fold from Escherichia coli by nickel affinity chromatography to homogeneity
recombinant wild-type and mutant enzymes from Escherichia coli strain JM109
-
recombinnat GAC from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography
using Ni-NTA chromatography
-