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3.5.1.2: glutaminase

This is an abbreviated version!
For detailed information about glutaminase, go to the full flat file.

Word Map on EC 3.5.1.2

Reaction

L-glutamine
+
H2O
=
L-glutamate
 +
NH3

Synonyms

AnsB, AoGls, GA, GAB, GAC, GahB, GLA, GLNase, GLS, GLS1, GLS2, GlsA, glutaminase, glutaminase 1, glutaminase 2, glutaminase A, glutaminase B, glutaminase C, glutaminase I, glutaminase K, glutaminase L, glutaminase-1, glutaminase-2, glutaminase-B, glutamine amidohydrolase, glutamine aminohydrolase, glutamine deamidating enzyme, K-glutaminase, KAG, KGA, kidney-type glutaminase, kidney-type-glutaminase, L-glutaminase, L-glutamine amidohydrolase, LAG, LGA, liver-type glitaminase, liver-type glutaminase, Mglu, Micrococcus glutaminase Mglu, Micrococcus luteus K-3-type glutaminase, mitochondrial glutaminase, N-PAG, neuroblastoma glutaminase, neuroblastoma PAG, Nit 2, nitrilase 2, omega-amidase, PAG, PDX2, phosphate activated glutaminase, phosphate-activated glutaminase, phosphate-activated L-glutamine amidohydrolase, salt-tolerant glutaminase, YaaE

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.2 glutaminase

Cloned

Cloned on EC 3.5.1.2 - glutaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Aspergillus oryzae strain RIB40 RP-1
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3)-RIL-Codon plus cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli C41(DE3)pLysS cells
-
expression analysis of glutaminase isoenzymes in tumor cells by competitive RT-PCR, overview
-
expression in Escherichia coli
expression in Escherichia coli strain JM109
-
expression in yeast strain EGY48
expression of FLAG-tagged KGA in 293T cells
expression of GAC in Spodoptera frugiperda Sf9 cells
expression of HA-tagged or FLAG-tagged wild-type and mutant kidney-type glutaminase in 293T cells
-
expression of wild-type and mutant enzymes in Escherichia coli strain JM109
-
gene AoglsA, from strain RIB40, DNA and amino acid sequence determination and analysis, functional overexpression in Saccharomyces cerevisiae cell wall and in Escherichia coli as soluble protein
gene CagahA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Saccharomyces cerevisiae strain INVSc
-
gene CngahA, a single copy gene in the chromosomal DNA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Saccharomyces cerevisiae strain INVSc
-
gene glsA, expression of His10-tagged GlsA in Escherichia coli under the control of a ptac promoter
gene prgA, overexpression of C-terminally His6-tagged wild-type and mutant A47Q enzymes in Escherichia coli strain BL21(DE3) cytosol
-
gene trpG, expression of His-tagged wild-type TrpG and mutant TrpG variants T129F and T129A in Escherichia coli strain BL21(DE3)Rosetta
-
GLS1 gene, cloning from genomic library, quantitative expression analysis
-
heterologously expressed in Lactobacillus plantarum KLB213 which exhibits a high salt-tolerance and Lactobacillus paracasei KLB58 which produces high amount of exopolysaccharide
-
high-expression in Escherichia coli
-
human L-type isoform expressed in baculovirus yields functional recombinant enzyme in Sf9 insect cells. Recombinant human glutaminase is targeted to both mitochondria and nucleus, and in both locations the protein is catalytically active
-
mouse nitrilase 2 is expressed as a His-tagged fusion protein in Escherichia coli and in HEK-293T cells
-
overexpression in Escherichia coli
-
overexpression of GLS2 reduces the growth of tumor cells and colony formation
-
the L gene, encoding the liver-type isozyme, is cloned from a brain library and is located on chromosome 12, while the kidney-type isozy
-