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3.5.1.16: acetylornithine deacetylase

This is an abbreviated version!
For detailed information about acetylornithine deacetylase, go to the full flat file.

Word Map on EC 3.5.1.16

Reaction

N2-Acetyl-L-ornithine
+
H2O
=
acetate
+
L-ornithine

Synonyms

acetylornithinase, AO, AOase, arg-E encoded N-acetyl L-ornithine deacetylase, ArgE, argE-encoded N-acetyl-L-ornithine deacetylase, At4g17830, E. coli AO, enterobacterial AOase, N(alpha)-acetyl-L-ornithine deacetylase, N-acetyl-L-ornithine deacetylase, N-acetylornithinase, N-acetylornithine deacetylase, N2-acetylornithine:N-acetylglutamate acetyltransferase, NAO, NAO deacetylase, NAOD, NAOGAcT

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.16 acetylornithine deacetylase

Cloned

Cloned on EC 3.5.1.16 - acetylornithine deacetylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
argE gene encoding enzyme cloned and sequenced, M13
Escherichia coli transformed with pTrc99A + pC-28 containing argE, lambdaZAP library from 2674
expression in Escherichia coli K-12. Because the purified 6His-Sumo-ArgE loses all activity following removal of the affinity tag, it is not possible to characterize the tag-free enzyme
gene argE, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21, wild-type and mutant H355A are soluble, the other mutants are insoluble
-
gene At4g17830, located on chromosome IV and organized into nine exons, which encodes two putative isozymes, UniProt IDs F4JPZ7 and Q9C5C4, sequence comparison and phylogenetic analysis
PCR-amplified argE, cloned and expressed
-
plasmid pMC7
-