3.5.1.15: aspartoacylase

This is an abbreviated version!
For detailed information about aspartoacylase, go to the full flat file.

Reaction

Synonyms

acetyl-aspartic deaminase, Acylase II, aminoacylase 2, Aminoacylase II, aminoacylase-2, ASPA, aspartic acid acylase, Aspartoacylase, aspartoacylase II, hASPA, human ASPA, More, murine aspartoacylase, N-acetyl-aspartate deacetylase, N-Acetyl-L-aspartate amidohydrolase, N-Acetylaspartate amidohydrolase, N-acetylaspartocylase, NAA acylase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.15 aspartoacylase

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Results	in table
26	Activating Compound
712	AA Sequence
7	Application
1	CAS Registry Number
17	Cloned(Commentary)
5	Crystallization (Commentary)
540	Disease/ Diagnostics
1	Expression
15	General Information
3	General Stability
14	Inhibitors
5	kcat/KM [mM/s]
1	Ki Value [mM]
25	KM Value [mM]
16	Localization
16	Metals/Ions
12	Molecular Weight [Da]
60	Natural Substrates/ Products (Substrates)
255	Organism
1	Oxidation Stability
3	Pathway
20	PDB ID
8	pH Optimum
1	pH Range
2	Posttranslational Modification
65	Protein Variants
16	Purification (Commentary)
2	Reaction
1	Reaction Type
73	Reference
1	Renatured (Commentary)
110	Source Tissue
11	Specific Activity [micromol/min/mg]
4	Storage Stability
164	Substrates and Products (Substrate)
11	Subunits
51	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Stability [°C]
13	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.5.1.15 - aspartoacylase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, frozen storage of undialyzed enzyme for a few days leads to a complete disappearance of activity following a single thaw

Rattus norvegicus

-

209000

-70°C, kept for up to 4 weeks without loss of activity

Homo sapiens

-

209008

4°C, purified wild-type enzyme, expressed

Homo sapiens

-

667727

the purified enzyme expressed in Pichia pastoris is significantly more stable than the Escherichia coli-expressed enzyme, losing less than 10% of its catalytic activity after 2 weeks when stored under conditions where the Escherichia coli-expressed enzyme becomes completely inactivated within 24 h, the Pichia pastoris-expressed enzyme shows sensitivity to oxidation over time and will precipitate if not kept under the proper reducing conditions

Homo sapiens

-

667727