3.5.1.136: N,N'-diacetylchitobiose non-reducing end deacetylase

This is an abbreviated version!
For detailed information about N,N'-diacetylchitobiose non-reducing end deacetylase, go to the full flat file.

Reaction

Synonyms

CDA, Dac, diacetylchitobiose deacetylase, N,N'-diacetylchitobiose deacetylase, NAc2 deacetylase, PH0499, Tk-Dac

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.136 N,N'-diacetylchitobiose non-reducing end deacetylase

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Results	in table
3	AA Sequence
8	Application
7	Cloned(Commentary)
4	Crystallization (Commentary)
1	Expression
1	Inhibitors
3	kcat/KM [mM/s]
5	KM Value [mM]
1	Localization
1	Metals/Ions
2	Molecular Weight [Da]
21	Natural Substrates/ Products (Substrates)
44	Organism
1	Pathway
2	pH Optimum
10	Protein Variants
5	Purification (Commentary)
1	Reaction
17	Reference
36	Substrates and Products (Substrate)
15	Subunits
24	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]

Substrates Products

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Substrates Products on EC 3.5.1.136 - N,N'-diacetylchitobiose non-reducing end deacetylase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

24 entries

N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Thermococcus kodakarensis

Q6F4N1

-

759461

-

-

?

N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Thermococcus kodakarensis

Q6F4N1

-

759461

-

-

?

N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Thermococcus kodakarensis

Q6F4N1

-

759461

-

-

?

N-acetyl-D-glucosamine + H2O

beta-D-glucosamine + acetate

3 entries

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus furiosus

Q8U3V1

-

759170

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus furiosus ATCC 43587

Q8U3V1

-

759170

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii

O58235

-

752555, 759170, 760088, 760232

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii

O58235

chitin degradation pathway in hyperthermophilic archaea

760232

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii

O58235

the enzyme is involved in the archaea-specific chitinolytic pathways by deacetylating the disaccharide end product of chitinase

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii

O58235

the crystal structure of the enzyme in complex with 2-deoxy-2-methylphosphoramido-D-glucose demonstrates that Arg92, Asp115, and His152 side chains interact with hydroxyl groups of the glucose moiety of the non-reducing-end GlcNAc residue

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii ATCC 700860

O58235

the enzyme is involved in the archaea-specific chitinolytic pathways by deacetylating the disaccharide end product of chitinase

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii ATCC 700860

O58235

the crystal structure of the enzyme in complex with 2-deoxy-2-methylphosphoramido-D-glucose demonstrates that Arg92, Asp115, and His152 side chains interact with hydroxyl groups of the glucose moiety of the non-reducing-end GlcNAc residue

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii ATCC 700860

O58235

-

752555, 759170, 760088, 760232

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii ATCC 700860

O58235

chitin degradation pathway in hyperthermophilic archaea

760232

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii DSM 12428

-

the enzyme is involved in the archaea-specific chitinolytic pathways by deacetylating the disaccharide end product of chitinase

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii DSM 12428

-

the crystal structure of the enzyme in complex with 2-deoxy-2-methylphosphoramido-D-glucose demonstrates that Arg92, Asp115, and His152 side chains interact with hydroxyl groups of the glucose moiety of the non-reducing-end GlcNAc residue

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii DSM 12428

-

-

752555

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii JCM 9974

-

the enzyme is involved in the archaea-specific chitinolytic pathways by deacetylating the disaccharide end product of chitinase

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii JCM 9974

-

the crystal structure of the enzyme in complex with 2-deoxy-2-methylphosphoramido-D-glucose demonstrates that Arg92, Asp115, and His152 side chains interact with hydroxyl groups of the glucose moiety of the non-reducing-end GlcNAc residue

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii JCM 9974

-

-

752555

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii NBRC 100139

-

the enzyme is involved in the archaea-specific chitinolytic pathways by deacetylating the disaccharide end product of chitinase

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii NBRC 100139

-

the crystal structure of the enzyme in complex with 2-deoxy-2-methylphosphoramido-D-glucose demonstrates that Arg92, Asp115, and His152 side chains interact with hydroxyl groups of the glucose moiety of the non-reducing-end GlcNAc residue

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii NBRC 100139

-

-

752555

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii OT-3

-

the enzyme is involved in the archaea-specific chitinolytic pathways by deacetylating the disaccharide end product of chitinase

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii OT-3

-

the crystal structure of the enzyme in complex with 2-deoxy-2-methylphosphoramido-D-glucose demonstrates that Arg92, Asp115, and His152 side chains interact with hydroxyl groups of the glucose moiety of the non-reducing-end GlcNAc residue

745652

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Pyrococcus horikoshii OT-3

-

-

752555

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Thermococcus kodakarensis

Q6F4N1

-

759461

-

-

?

N,N'-diacetylchitobiose + H2O

beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate

Thermococcus kodakarensis

Q6F4N1

the enzyme is involved in the chitin catabolic pathway

759461

-

-

?

N-acetyl-D-glucosamine + H2O

beta-D-glucosamine + acetate

Pyrococcus furiosus

Q8U3V1

-

754411

-

-

?

N-acetyl-D-glucosamine + H2O

beta-D-glucosamine + acetate

Pyrococcus furiosus ATCC 700860

Q8U3V1

-

754411

-

-

?

N-acetyl-D-glucosamine + H2O

beta-D-glucosamine + acetate

Thermococcus kodakarensis

Q6F4N1

-

759461

-

-

?