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3.5.1.136: N,N'-diacetylchitobiose non-reducing end deacetylase

This is an abbreviated version!
For detailed information about N,N'-diacetylchitobiose non-reducing end deacetylase, go to the full flat file.

Reaction

N,N'-diacetylchitobiose
+
H2O
=
beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine
 +
acetate

Synonyms

CDA, Dac, diacetylchitobiose deacetylase, N,N'-diacetylchitobiose deacetylase, NAc2 deacetylase, PH0499, Tk-Dac

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.136 N,N'-diacetylchitobiose non-reducing end deacetylase

Cloned

Cloned on EC 3.5.1.136 - N,N'-diacetylchitobiose non-reducing end deacetylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amplified by polymerase chain reaction, cloned into expression vector pET15b, and expressed in E. coli BL21-codonPlus (DE3)-RIL
expression in Bacillus subtilis
expression in Bacillus subtilis WB600 is better than expression in Escherichia coli BL21(DE3)
expression in Escherichia coli
no expression in the soluble fraction of Escherichia coli harboring the expression plasmid. Insertion of the target genes into the chromosome of Escherichia coli yields the soluble recombinant protein
overexpressed as inclusion bodies in Escherichia coli Rosetta (DE3) pLys and successfully refolded in vitro