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3.5.1.12: biotinidase

This is an abbreviated version!
For detailed information about biotinidase, go to the full flat file.

Word Map on EC 3.5.1.12

Reaction

Biotin amide
+
H2O
=
biotin
+
NH3

Synonyms

amidohydrolase biotinidase, biocytin hydrolyzing amidase, biotinidase, biotinyl-hydrolase, BTD, lipoamidase, lipolysine hydrolase, lipoyl-X-hydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.12 biotinidase

Storage Stability

Storage Stability on EC 3.5.1.12 - biotinidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, activity decreases upon storage
-
-20°C, mercaptoethanol, a few months without loss of activity
-
-20°C, phosphate buffer pH 7.5, a few months
-
-70°C stable
-
15°C enzyme solution with toluene, for a week or more without appreciable loss of activity
-
4°C, 0.1 M phosphate buffer pH 6.0, 1 mM 2-mercaptoethanol, 1 mM EDTA, 1 month without loss of activity
-