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3.5.1.12: biotinidase

This is an abbreviated version!
For detailed information about biotinidase, go to the full flat file.

Word Map on EC 3.5.1.12

Reaction

Biotin amide
+
H2O
=
biotin
+
NH3

Synonyms

amidohydrolase biotinidase, biocytin hydrolyzing amidase, biotinidase, biotinyl-hydrolase, BTD, lipoamidase, lipolysine hydrolase, lipoyl-X-hydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.12 biotinidase

Molecular Weight

Molecular Weight on EC 3.5.1.12 - biotinidase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
115000
56770
-
estimated from amino acid sequence
60000
-
gel filtration, treated with N-glycanase
66000 - 76000
68000
70000
-
gel permeation, nondenaturating
70000 - 80000
-
glycosylated enzyme
71000
-
gel filtration, treated with neuraminidase
76000
76500
-
gel filtration, SDS-PAGE, normal serum biotinidase has 9 isoforms
78000
-
gel filtration
80000
-
SDS-PAGE
85000
-
-
92000
-
gel filtration