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3.4.24.70: oligopeptidase A

This is an abbreviated version!
For detailed information about oligopeptidase A, go to the full flat file.

Word Map on EC 3.4.24.70

Reaction

hydrolysis of oligopeptides, with broad specificity. Gly or Ala commonly occur as P1 or P1' residues, but additional information distant residues are also important, as is shown by the fact that Z-Gly-Pro-Gly-/-Gly-Pro-Ala is cleaved, but not Z-(Gly)5 =

Synonyms

68000-M Signalpeptide hydrolase, More, oligopeptidase A, OpdA, prlC, TOP, Top1, Top2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.70 oligopeptidase A

Purification

Purification on EC 3.4.24.70 - oligopeptidase A

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia ocli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged isozyme from Escherichia coli BL21 Rosetta (DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged wild-type OpdA and mutants from Escherichia coli strain DH5alpha by nickel affinity chromatography
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to near homogeneity
-