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3.4.24.67: choriolysin H

This is an abbreviated version!
For detailed information about choriolysin H, go to the full flat file.

Word Map on EC 3.4.24.67

Reaction

Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-/-7-(4-methyl)coumarylamide =

Synonyms

More, Teleost hatching enzyme (component), High choriolytic enzyme, hatching enzyme, chorionase, HCE, HCE-1, embryo-specific hatching enzyme, medaka hatching enzyme, MHCE, PHE, HCE1, HCE2, ZHE1, FHCE1, FHCE2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.67 choriolysin H

Temperature Stability

Temperature Stability on EC 3.4.24.67 - choriolysin H

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 40
-
10 min, gradual decrease of choriolytic activity (10-30% loss)
25
-
4 days, in 50 mM Tris-HCl, 10 mM NaCl, pH 7.5, about 80% loss of choriolytic activity, 0.05% Brij-35 stabilizes, caseinolytic activity is less susceptible to long-term incubation
44
-
10 min, about 50% loss of choriolytic activity
50
-
10 min, about 90% loss of choriolytic activity
60
-
inactivation