3.4.24.67: choriolysin H

This is an abbreviated version!
For detailed information about choriolysin H, go to the full flat file.

Word Map on EC 3.4.24.67

Reaction

Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-/-7-(4-methyl)coumarylamide =

Synonyms

More, Teleost hatching enzyme (component), High choriolytic enzyme, hatching enzyme, chorionase, HCE, HCE-1, embryo-specific hatching enzyme, medaka hatching enzyme, MHCE, PHE, HCE1, HCE2, ZHE1, FHCE1, FHCE2

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.67 choriolysin H

Substrates Products

Substrates Products on EC 3.4.24.67 - choriolysin H

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Benzoyl-Arg 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
hydrolysis at about 3% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
about 8.5% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
benzyloxycarbonyl-Phe + Arg-4-methylcoumarin 7-amide
show the reaction diagram
-
-
-
-
?
Benzyloxycarbonyl-Phe-Ser-Arg 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
hydrolysis at about 2% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
benzyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O
?
show the reaction diagram
-
preferred by HCE1
-
-
?
casein + H2O
?
show the reaction diagram
chorion + H2O
?
show the reaction diagram
Chorion + H2O
Swollen chorion + ?
show the reaction diagram
EVLPLDNPPPA + H2O
EVLPLD + NPPPA
show the reaction diagram
GSKTPQDPTQQ + H2O
GSKTPQ + DPTQQ
show the reaction diagram
-
location: Q39/D40 in ChgL (ZI-3). Specific activity: 28.5 nmol/30min/microgram enzyme
-
-
?
KLMLKAPEPF + H2O
KLMLK + APEPF
show the reaction diagram
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 7.6% of the rate with TVQQSDYLIK
-
-
?
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
hydrolysis at about 12% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
NPQVPQYPSKPQ + H2O
NPQVPQ + YPSKPQ
show the reaction diagram
NPSYPQNPSYPQ + H2O
NPSYPQ + NPSYPQ
show the reaction diagram
Peptidyl 4-methylcoumarin 7-amides + H2O
?
show the reaction diagram
-
i.e. MCA-peptides
-
-
?
PGKNPNTPPIG + H2O
PGKNPN + TPPIG
show the reaction diagram
-
location: N220/T221 in ChgH (ZI-1,2). Specific activity: 11.3 nmol/30min/microgram enzyme
-
-
?
PLPVRVEEVV + H2O
PLPVR + VEEVV
show the reaction diagram
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein. Cleavage at 38% of the rate with TVQQSDYLIK
-
-
?
PSKRPEAPGVP + H2O
PSKRPE + APGVP
show the reaction diagram
QTPSYPQQ + H2O
QTPS + YPQQ
show the reaction diagram
SAPVVRTSQAV + H2O
NPQVPQ + YPSKPQ
show the reaction diagram
-
synthetic substrate generated from the 25 kDa product in hatching liquid with an inefficient cleavage site
-
-
?
SKPQYPNP + H2O
SKPQ + YPNP
show the reaction diagram
Suc-Leu-Leu-Val-Tyr-4-methylcoumarin-7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
Succinyl-Ala-Ala-Pro-Phe 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
hydrolysis at about 13% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
Succinyl-Ala-Pro-Ala 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
hydrolysis at about 45% the rate of succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide
-
-
?
succinyl-Ala-Pro-Ala-4-methylcoumaryl-7-amide + H2O
?
show the reaction diagram
-
best substrate for both isoforms of HCE
-
-
?
succinyl-Ile-Ile-Trp-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
about 5.7% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
Succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide + H2O
Succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
best substrate of peptidyl 4-methylcoumarin 7-amides
-
-
?
succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
about 40% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide + H2O
?
show the reaction diagram
-
preferred by HCE2
-
-
?
t-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
about 9% of the rate with benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
-
?
TVQQSDYLIK + H2O
TVQQS + DYLIK
show the reaction diagram
-
synthetic peptide substrate designed according to cleavage site in egg envelope protein
-
-
?
VPFELRYPVPA + H2O
VPFELR + YPVPA
show the reaction diagram
-
specific activity: 0.05 nmol/min/microgram protein
-
-
?
YPSKPQTPTET + H2O
YPSKPQ + TPTET
show the reaction diagram
ZP2 + H2O
?
show the reaction diagram
-
zebrafish egg envelope glycoprotein, cleavage at Ser47/Asp48 and Arg66/Val67 in the unfertilized egg, at Gln79/Ala80 in the fertilized egg
-
-
?
ZP3 + H2O
?
show the reaction diagram
-
zebrafish egg envelope glycoprotein, cleavage at Gln79/Ala80 in the unfertilized egg, at Gln67/Ala68 in the fertilized egg
-
-
?
additional information
?
-