3.4.24.67: choriolysin H
This is an abbreviated version!
For detailed information about choriolysin H, go to the full flat file.
Reaction
Hydrolysis of the inner layer of fish egg envelope. Also hydrolysis of casein and small molecule substrates such as succinyl-Leu-Leu-Val-Tyr-/-7-(4-methyl)coumarylamide =
Synonyms
chorionase, embryo-specific hatching enzyme, FHCE1, FHCE2, hatching enzyme, HCE, HCE-1, HCE1, HCE2, High choriolytic enzyme, medaka hatching enzyme, MHCE, More, PHE, Teleost hatching enzyme (component), ZHE1
ECTree
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Inhibitors
Inhibitors on EC 3.4.24.67 - choriolysin H
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Anti-HCE antibody
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inhibits choriolytic swelling and binding to chorion, no effect on caseinolytic activity
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antichoriolysin H
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inhibits both choriolytic activity and binding to the egg envelope, does not affect caseinolytic activity
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EDTA
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succinyl-Leu-Leu-Val-Tyr 4-methylcoumarin 7-amide as substrate, 0.8 mM ZnCl2 restores or 80 mM CuCl2 partially restores, not NiCl2, MgCl2 or CaCl2
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not inhibitory: diisopropylfluorophosphate, iodoacetic acid
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additional information
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no inhibition by monoiodoacetamide, monoiodoacetic acid or diisopropyl fluorophosphate
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additional information
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not inhibited by DFP, iodoacetamide or iodoacetic acid
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additional information
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the enzyme is very sensitive to trypsin-specific inhibitors, especially serine protease-specific inhibitors
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