Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.24.63: meprin B

This is an abbreviated version!
For detailed information about meprin B, go to the full flat file.

Word Map on EC 3.4.24.63

Reaction

Hydrolysis of proteins, including azocasein, and peptides. Hydrolysis of -His5-/-Leu-, -Leu6-/-Cys-, -Ala14-/-Leu- and -Cys19-/-Gly- bonds in insulin B chain =

Synonyms

beta-secretase, cell surface sheddase, h-meprin beta, MEP1B, mephrin beta, meprin A subunit beta, Meprin b, meprin B metalloprotease, meprin beta, meprin beta metalloproteinase, meprin metalloprotease, meprin metalloproteinase, meprin metalloproteinase beta, meprin-beta, meprinbeta, metalloprotease meprin, metalloprotease meprin B, metalloprotease meprin beta, metalloproteinase meprin beta, Mmepb, More, mouse meprin beta, procollagen proteinase, Rmepb

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.63 meprin B

Cloned

Cloned on EC 3.4.24.63 - meprin B

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
4 genes encoding alpha and beta subunits of the enzyme are located on chromosome 20, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, sequence comparisons, overview
analysis of expression level of meprinbeta in high IgA mouse (time dependent)
-
expression of mepbetaDELTATM, meprin beta coding region lacking the transmembrane and intracellular domains with V5 and hexahistidine C-terminal tags (termed as mepbetaDELTATM) in HEK-293F cells
expression of the soluble tail switch mutant pro-meprinbeta in BT1-TN-5B1-4 insect cells using the baculovirus transfection system
-
gene MEP1B
gene MEP1B, recombinant enzyme expression in COS-7 and HEK293T cells
gene MEP1B, recombinant expression of C-terminally or N-terminally His6-tagged enzyme in Pichia pastoris strain X33 or SMD1168H. The N-terminal signal sequence is replaced by the alpha-leader of Saccharomyces, enabling efficient secretion of the mature enzyme, harboring either an N-terminal or C-terminal His-tag. The recombinant enzyme is N-glycosylated and secreted as a dimer
gene MEP1B, recombinant expression of meprin beta in Pichia pastoris strain X-33
gene Mep1B, recombinant expression of N-terminally His-tagged meprin beta in HEK-293 cells, recombinant expression of meprin beta in COS-7 cells and coexpression with matrilysin-2 or pancreatic trypsin
gene MEP1B, recombinant expression of N-terminally His6-tagged meprin beta Pichia pastoris strain X33
gene MEP1B, recombinant expression of wild-type and mutant enzymes in HEK-293 cells
gene Mep1B, sequence comparisons
gene Mep1B, transient overexpression of meprin beta in MEF cells, cell surface localization
homooligomeric meprin B produced by transfecting cells with alpha or beta cDNA
-
Mep1B, recombinant expression of homomeric meprin B used in human embryonic kidney (HEK293) cells, coexpression with meprin A (EC 3.4.24.18), recombinant expression of meprin B in MEDCK cells
mouse, Mep-1beta structural gene, localized to chromosome 18
-
rat, expressed in human kidney cell line 293, the expressed protein displays no detectable peptidase activity unless it is activated by removal of amino-terminal prosequence by limited trypsin digestion
-
recombinant expression of C-terminally Flag-tagged meprin beta in HEK-293 cells
recombinant expression of C-terminally His6-tagged meprin beta protein (1-648) in human HEK-293 cells
recombinant expression of FLAG-tagged enzyme mutants in HEK-293 and COS-7 cells, recombinant expression of N-terminally His-tagged human meprin beta zymogen in Hi5 insect cells via viral transfection method
recombinant expression of His-tagged enzyme in Spodoptera frugiperda Sf9 insect cells
recombinant expression of meprin beta in yeast
recombinant His-tagged enzyme, cDNA transfected into human embryonic kidney 293 cells
-
recombinantly expressed
-
transient co-expression of amyloid precursor protein APP751 and meprin beta in HEK293T cells. Generation of BACE1/2-KO mouse fibroblasts overexpressing human APP695 isoform and human meprin beta