3.4.24.62: magnolysin
This is an abbreviated version!
For detailed information about magnolysin, go to the full flat file.
Reaction
Hydrolysis of polypeptides with Arg or Lys in P1 and P2, e.g. to hydrolyse pro-oxytocin at -Lys-Arg-/-Ala-Val-. The specificity further depends on the organization of a beta-turn-alpha-helix of nine or additional information residues containing the paired basic amino acids near the centre =
Synonyms
Bovine neurosecretory granule protease cleaving pro-oxytocin/neurophysin, pro-ocytocin convertase, pro-ocytocin/neurophysin-converting enzyme, Pro-oxytocin convertase, Pro-oxytocin/neurophysin convertase, Proteinase, prooxyphysin
ECTree
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Substrates Products
Substrates Products on EC 3.4.24.62 - magnolysin
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REACTION DIAGRAM
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
[Arg11,Lys12]-Pro-oxytocin/neurophysin(1-20) + H2O
?
-
i.e. peptide VI, hydrolysis at the same rate as pro-oxytocin/neurophysin(1-20)
-
-
?
Pro-oxytocin/neurophysin + H2O
?
-
involved in post-translational processing
-
-
?
Pro-oxytocin/neurophysin + H2O
?
-
primary cleavage at the Arg12-Ala13 bond
-
-
?
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
obtained by hemisynthesis
i.e. peptide II (i.e. oxytocinGly10Lys11Arg12 and peptide III)
?
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
synthetic eicosapeptide reproducing the entire sequence of amino-terminal domain of bovine proOc/Np precursor
-
?
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
strictly selective for basic amino acid doublet Lys11-Arg12, cleavage site: Arg12-Ala13
-
?
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
strictly selective for basic amino acid doublet Lys11-Arg12, cleavage site: Arg12-Ala13
-
?
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
strictly selective for basic amino acid doublet Lys11-Arg12, cleavage site: Arg12-Ala13
i.e. peptide II (i.e. oxytocinGly10Lys11Arg12 and peptide III)
?
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
strictly selective for basic amino acid doublet Lys11-Arg12, cleavage site: Arg12-Ala13
i.e. peptide II (i.e. oxytocinGly10Lys11Arg12 and peptide III)
?
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
i.e. model substrate peptide I
-
?
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
i.e. model substrate peptide I
-
?
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
i.e. model substrate peptide I
-
-
?
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
i.e. model substrate peptide I
i.e. peptide II (i.e. oxytocinGly10Lys11Arg12 and peptide III)
?
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
i.e. model substrate peptide I
i.e. peptide II (i.e. oxytocinGly10Lys11Arg12 and peptide III)
?
Pro-oxytocin/neurophysin(1-20) + H2O
Pro-oxytocin/neurophysin(1-12) + pro-oxytocin/neurophysin(13-20)
-
strictly selective for basic amino acid doublet Lys11-Arg12, cleavage site: Arg12-Ala13
i.e. peptide II (i.e. oxytocinGly10Lys11Arg12 and peptide III)
?
[Ala10]-Pro-oxytocin/neurophysin(1-20) + H2O
?
-
i.e. peptide XI, hydrolysis at the same rate as pro-oxytocin/neurophysin(1-20)
-
-
?
additional information
?
-
-
relationship between structure and enzyme action
-
-
?
additional information
?
-
-
relationship between structure and enzyme action
-
-
?
additional information
?
-
-
specificity depends on the organization of a beta-turn-alpha-helix of nine or more residues containing the basic amino acid doublet near the centre
-
-
?
additional information
?
-
-
structural requirements of the substrate peptides (pro-OT/Np-model)
-
-
?
additional information
?
-
-
No substrates are pro-oxytocin/neurophysin peptides (i.e. pro-OT/Np(1-20)) with D-Arg12
-
-
?
additional information
?
-
-
No substrates are pro-oxytocin/neurophysin peptides (i.e. pro-OT/Np(1-20)) with D-Arg12
-
-
?
additional information
?
-
-
No substrates are pro-oxytocin/neurophysin peptides (i.e. pro-OT/Np(1-20)) with D-Arg12
-
-
?
additional information
?
-
-
peptides bearing Nle or D-Arg at subsite P1 or containing Nle or D-Lys at subsite P2 remain uncleaved, substrate bearing the Lys-Arg doublet is hydrolyzed with best efficiency, a minimal length of eight residues is necessary to allow for substrate binding
-
-
?
additional information
?
-
-
peptides bearing Nle or D-Arg at subsite P1 or containing Nle or D-Lys at subsite P2 remain uncleaved, substrate bearing the Lys-Arg doublet is hydrolyzed with best efficiency, a minimal length of eight residues is necessary to allow for substrate binding
-
-
?