3.4.24.50: bothrolysin

This is an abbreviated version!
For detailed information about bothrolysin, go to the full flat file.

Cleavage of Gln4-/-His, Ser9-/-His and Ala14-/-Leu of insulin B chain and Pro-/-Phe of angiotensin I =

Bothrops metalloendopeptidase J, J Protease

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Results	in table
1	AA Sequence
1	CAS Registry Number
10	Inhibitors
2	Metals/Ions
2	Molecular Weight [Da]
2	Organism
1	pH Optimum
2	Purification (Commentary)
1	Reaction
1	Reaction Type
2	Reference
2	Source Tissue
5	Substrates and Products (Substrate)
1	Subunits
3	Synonyms
1	Temperature Optimum [°C]

Inhibitors on EC 3.4.24.50 - bothrolysin

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1,10-phenanthroline

5,5'-dithiobis(2-nitrobenzoic acid)

1 mM: 70% inhibition, in presence of 2 mM Ca2+: 30% inhibition

DM43

serum from opossum, inhibits the fibrinogenolytic activity of bothrolysin and forms a stable complex with it

DTNB

inhibitory, partially prevented by addition of Ca2+

EDTA

Protein inhibitor

from blood plasma of Bothrops jararaca, completely inhibits

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