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3.4.24.35: gelatinase B

This is an abbreviated version!
For detailed information about gelatinase B, go to the full flat file.

Word Map on EC 3.4.24.35

Reaction

Cleavage of gelatin types I and V and collagen types IV and V =

Synonyms

92 kDa gelatinase, 92 kDa type IV collagenase, 92 kDa type IV collagenase proMMP-9, 92-kDa Gelatinase, 92-kDa gelatinase B, 92-kDa Type IV collagenase, 95 kDa type IV collagenase/gelatinase, Collagenase IV, Collagenase type IV, gelatinase, gelatinase B, Gelatinase MMP 9, gelatinolytic enzyme, GELB, Macrophage gelatinase, matrix metallopeptidase 9, matrix metallopeptidase 9 gelatinase B, matrix metalloprotease-9, Matrix metalloproteinase 9, matrix metalloproteinase-9, metrix metalloproteinase-9, MMP 9, MMP-9, MMP9, neutrophil collagenase, pro-matrix metalloproteinase-9, progelatinase, Type IV collagen metalloproteinase, Type IV collagenase, Type IV collagenase/gelatinase, Type V collagenase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.35 gelatinase B

Purification

Purification on EC 3.4.24.35 - gelatinase B

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
catalytic domain of mutated enzyme
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gelatin Sepharose 4B bead chromatography
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gelatin-Sepharose column chromatography
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native enzyme from serum samples by concanavalin-A and gelatin afffinity chromatography, and ultrafiltration
native enzyme from tibia by gelatin-agarose affinity chromatography, anion exchange chromatography, and dialysis
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native MMP-9 from dentin inculding serial dilutions, method development
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native MMP-9 from gastric mucosa
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purification, refolding and in vitro recovery of active full length recombinant human gelatinase MMP-9
recombinant activated MMP-9 dimers by gelatin affnity chromatography
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recombinant enzyme
recombinant full-length pro-MMP-9 from Spodoptera frugiperda Sf9 cells
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recombinant full-length proMMP-9 and mutant enzymes from Spodoptera frugiperda Sf9 insect cells
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recombinant MMP-9 from Spodoptera frugiperda Sf9 cells
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recombinant secreted mutant enzymes from Hep-G2 cells by gelatin affinity chromatography to homogeneity
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recombinant wild-type and mutant zymogen from Sf9 insect cells by gelatin affinity chromatography
SC40-transformed cells
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