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3.4.24.35: gelatinase B

This is an abbreviated version!
For detailed information about gelatinase B, go to the full flat file.

Word Map on EC 3.4.24.35

Reaction

Cleavage of gelatin types I and V and collagen types IV and V =

Synonyms

92 kDa gelatinase, 92 kDa type IV collagenase, 92 kDa type IV collagenase proMMP-9, 92-kDa Gelatinase, 92-kDa gelatinase B, 92-kDa Type IV collagenase, 95 kDa type IV collagenase/gelatinase, Collagenase IV, Collagenase type IV, gelatinase, gelatinase B, Gelatinase MMP 9, gelatinolytic enzyme, GELB, Macrophage gelatinase, matrix metallopeptidase 9, matrix metallopeptidase 9 gelatinase B, matrix metalloprotease-9, Matrix metalloproteinase 9, matrix metalloproteinase-9, metrix metalloproteinase-9, MMP 9, MMP-9, MMP9, neutrophil collagenase, pro-matrix metalloproteinase-9, progelatinase, Type IV collagen metalloproteinase, Type IV collagenase, Type IV collagenase/gelatinase, Type V collagenase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.35 gelatinase B

Cloned

Cloned on EC 3.4.24.35 - gelatinase B

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA encoding the catalytic domain cloned into pCDNA3 and used to transfect HEK 293 cells
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cDNA of the mutated enzyme used to transfect HEK 293 cells
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expressed in A549-C and A549-FI cells
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expressed in baculovirus-infected Spodoptera frugiperda IPLB-Sf21 AE cells
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expressed in CT-26 cells
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expressed in immortalized keratinocytes, transduced by lentivrus
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expression in H9C2 cell
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expression in the form of inclusion bodies in Escherichia coli BL21, using pET21a vector
expression of full-length pro-MMP-9 in Spodoptera frugiperda Sf9 cells
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expression of full-length proMMP-9 and of mutant enzymes in Spodoptera frugiperda Sf9 insect cells using the baculovirus infection system
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expression of MMP-9 in Spodoptera frugiperda Sf9 cells
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expression of mutant enzymes in Hep-G2 cells using recombinant adenoviral vectors adenovirus-2 or adenovirus-5, secretion of the recombinant enzymes, overview
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expression of wild-type full-length MMP-9 and of a mutant version lacking the O-glycosylated linker region and hemopexin domains in Drosophila melanogaster S2 cells using the Drosophila metallothionein promoter, the native MMP-9 signal peptide is utilized for secretion contained in the beginning of the sequences
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expresssion of wild-type MMP-9 and truncated versions in insect cells
gene MMP-9, quantitative real time PCR expression analysis in glioblastoma
gene MMP9, cloning of an enzyme fragment, containing an ATG start codon, signal peptide, prodomain sequence, active site, fibronectin II repeat domain and zinc binding sequence,from chondrocyte cDNA, stable recombinant expression in a rat chondrosarcoma cell line
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gene MMP9, quantitative expression analysis
gene MMP9, quantitative real-time PCR enzyme expression analysis
gene MMP9, quantitative RT-PCR enzyme expression analysis
recombinant expression of the wild-type and mutant zymogen in Spodoptera frugiperda Sf9 cells