3.4.24.3: microbial collagenase

This is an abbreviated version!
For detailed information about microbial collagenase, go to the full flat file.

Word Map on EC 3.4.24.3

Reaction

Digestion of native collagen in the triple helical region at -/-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3' =

Synonyms

More, collagenase, aspergillopeptidase C, azocollase, clostridiopeptidase A, clostridiopeptidase I, clostridiopeptidase II, collagen peptidase, collagenase A, collagenase MMP-1, EC 3.4.4.19, EC 3.4.99.5, kollaza, matrix metalloproteinase-1, metallocollagenase, metalloproteinase-1, MMP-1, nucleolysin, peptidase, clostridio-, A, proteinase, Clostridium histolyticum, A, soycollagestin, collagen protease, matirx metalloproteinase-18, collagenase I, Clostridium histolyticum collagenase, Achromobacter iophagus collagenase, 120 kDa collagenase, ColG, ChC, bacterial collagenase, thermophilic collagenolytic protease, collagenase G, clostridial collagenase A, CCA, collagenase H, ColH, collagenase T, ColT, microbial collagenase, M9-peptidase, class II collagenase, bacterial collagenase V, ColA, class III collagenase, VMC peptidase, BC_2466, class I collagenase, clostridial collagenase, type I collagenase, Dupuytren collagenase, Dupuytren clostridium histolyticum, collagenase clostridium histolyticum, type II collagenase, metalloproteinase ColB, Clostridium histolyticum class II collagenase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.3 microbial collagenase

Storage Stability

Storage Stability on EC 3.4.24.3 - microbial collagenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, lyophilized enzyme: 5 years, solutions: several months
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37°C, loss of 10% activity after 24 h and of 30% after 48 h, crude commercial preparation, overview
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4°C, 24 h, pH 4.4-7.4
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4°C, 24 h, plus 10 mM cysteine
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